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Abstract

A novel actinomycete, designated NUM-2625, was isolated as an endophytic bacterium in aerial parts of , an endemic species in the Altai, Himalaya mountain chain area, collected from Khasagt Khairkhan Mountain in Mongolia. The 16S rRNA gene sequence of strain NUM-2625 showed the highest similarity to TT2-10 (99.4 %), KV-744 (99.3 %), and CS5-AC17 (97.7 %). Chemotaxonomic properties of strain NUM-2625 were essentially consistent with those of the genus such as the presence of -diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, MK-9(H) and MK-9(H) as the major menaquinones, and iso-C, iso-C, iso-C 3-OH, and anteiso-C as the major fatty acids. Meanwhile, digital DNA–DNA hybridization and average nucleotide identity values revealed a low relatedness between strain NUM-2625 and the other type strains of the genus . In addition, strain NUM-2625 exhibited several phenotypic properties that could be used to distinguish it from its closest relatives. Based on the results of polyphasic analyses, strain NUM-2625 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NUM-2625 (=NBRC 114660=TBRC 13496).

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2021-07-09
2021-07-29
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