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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 6

gen. nov., sp. nov., a novel non-marine bacterium belonging to the group, and reclassification of (Zhong . 2015) as gen. nov., comb. nov. Free

Abstract

An aerobic, Gram-stain-negative, non-sporulating, flagellated and spindle-like bacterium, designated HY14, was isolated from a pickle-processing factory wastewater sample. The isolate chemoheterotrophically grew at 4–42 °C (optimum, 35 °C) and pH 5.5–9.0 (optimum, pH 6.0–6.5). Salt was required for growth (0.5–12 % NaCl, w/v). A deep brown and water-soluble uncharacterized pigment was produced when grown in certain media. The predominant fatty acids (>5 %) included C, C 7, 11-methyl C 7 and C cyclo 8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, two unidentified phospholipids, two unidentified glycolipids and five unknown lipids. The major isoprenoid quinone was ubiquinone-10. Pairwise alignment based on 16S rRNA gene sequences indicated that strain HY14 had the highest sequence similarity to genera (95.61–96.05 %) and (95.82 %). Phylogenetic analysis based on core genome illustrated that strain HY14 formed a monophyletic lineage with members of the genus in the clade of the group in the family . The core-gene average amino acid identity used to define bacterial genera by a threshold of 60–80 % was calculated to be 68.56–76.5 % between HY14 and closely related taxa. Several genomic characteristics, such as carrying two RuBisCO-mediated pathways and different osmoprotectant transport pathways, exhibited the genotypic discrepancies of strain HY14. Based on the polyphasic taxonomic characterization, strain HY14 is considered to represent a novel species of a novel genus belonging to the family , for which the name gen. nov., sp. nov. is proposed. The type strain is HY14 (=CGMCC 1.15973=KCTC 52499). (Zhong . 2015) is considered to diverge from at the genus level, and should be reassigned as a novel genus, for which the name gen. nov., comb. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Maritimibacter lacisalsi , Muriiphilus fusiformis , pickle , polyphasic taxonomy , reclassification and Roseobacter group
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31700566)
    • Principle Award Recipient: Xin-QiZhang
© 2021 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: gen. nov., sp. nov., a novel non-marine bacterium belonging to the group, and reclassification of (Zhong . 2015) as gen. nov., comb. nov.
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2021-06-28
2025-04-28
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Muriiphilus fusiformis gen. nov., sp. nov., a novel non-marine bacterium belonging to the Roseobacter group, and reclassification of Maritimibacter lacisalsi (Zhong et al. 2015) as Muriicola lacisalsi gen. nov., comb. nov.
Int J Syst Evol Microbiol 71, 004859 (2021); https://doi.org/10.1099/ijsem.0.004859
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/content/journal/ijsem/10.1099/ijsem.0.004859
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