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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 7

sp. nov. and sp. nov., isolated from bat faeces of and species No Access

Abstract

Four aerobic, Gram-stain-positive, rod-shaped bacteria (HY60, HY54, HY82 and HY89) were isolated from bat faeces of and species collected in PR China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the four novel strains formed two separate but adjacent subclades close to CGMCC 1.12260 (97.6–97.7 % similarity), JCM 18706 (97.3–97.5 %) and JCM 30493 (97.3–97.4 %). The 16S rRNA gene sequence similarity was 98.3 % between strains HY60 and HY82, and identical within strain pairs HY60/HY54 and HY82/HY89. The DNA G+C contents of strains HY60 and HY82 were 61.9 and 63.3 mol%, respectively. The digital DNA–DNA hybridization and average nucleotide identity values between each novel strain and their closest relatives were all below the 70 % and 95–96 % thresholds for species delimitation, respectively. All four novel strains contained -C, -C, -C and -C as the main fatty acids, MK-11 and MK-12 as the major respiratory quinones, and diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid as the predominant polar lipids. The cell-wall peptidoglycan was of B type and contained alanine, glutamate, glycine and ornithine. The acyl type of the muramic acid was glycolyl. The whole-cell sugars were rhamnose and ribose. Based on the foregoing polyphasic analyses, it was concluded that the four uncharacterized strains represented two novel species of the genus , for which the names sp. nov. [type strain HY60 (=CGMCC 1.17468=GDMCC 1.1951=KACC 22102)] and sp. nov. [type strain HY82 (=CGMCC 1.17469=GDMCC 1.1949=KACC 22101)] are proposed, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Hipposideros , Microbacterium chengjingii , Microbacterium fandaimingii and Rousettus
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2019YFC1200505)
    • Principle Award Recipient: JingYang
  • National Key R&D Program of China (Award 2019YFC1200500)
    • Principle Award Recipient: JingYang
  • National Science and Technology Major Project of China (Award 2017ZX10303405-005-002)
    • Principle Award Recipient: HanZheng
  • National Science and Technology Major Project of China (Award 2017ZX10303405-002)
    • Principle Award Recipient: HanZheng
  • National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: ShanLu
© 2021 The Authors
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2021-07-07
2024-04-24
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Microbacterium chengjingii sp. nov. and Microbacterium fandaimingii sp. nov., isolated from bat faeces of Hipposideros and Rousettus species
Int J Syst Evol Microbiol 71, 004858 (2021); https://doi.org/10.1099/ijsem.0.004858
/content/journal/ijsem/10.1099/ijsem.0.004858
/content/journal/ijsem/10.1099/ijsem.0.004858
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