An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Streptomyces. The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
PridhamTG, HesseltineCW, BenedictRG. A guide for the classification of streptomycetes according to selected groups; placement of strains in morphological sections. Appl Microbiol1958; 6:52–79 [PubMed]
KhebiziN, BoudjellaH, BijaniC, BourasN, KlenkH-P et al. Oligomycins A and E, major bioactive secondary metabolites produced by Streptomyces sp. strain HG29 isolated from a Saharan soil. J Mycol Med2018; 28:150–160 [View Article] [PubMed]
JangJ-P, HwangGJ, JangM, TakahashiS, KoS-K et al. Aturanosides A and B, glycosylated anthraquinones with antiangiogenic activity from a soil-derived Streptomyces species. J Nat Prod2018; 81:2004–2009
KemungHM, TanLT-H, ChanKG, SerHL, LawJW et al.Streptomyces sp. strain MUSC 125 from mangrove soil in Malaysia with anti-MRSA, anti-biofilm and antioxidant activities. Molecules2020; 25:3545
KellyKL. Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office; 1964
MinnikinDE, O’DonnellAG, GoodfellowM, AldersonG, AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241
CollinsMD, JonesD. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol1980; 48:459–470 [View Article]
CollinsMD, PirouzT, GoodfellowM, MinnikinDE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol1977; 100:221–230 [View Article] [PubMed]
KämpferP, KroppenstedtRM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol1996; 42:989–1005 [View Article]
TamaokaJ. Determination of DNA base composition. GoodfellowM, O’DonnellA. eds In Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994 pp 463–470
YoonS-H, HaS-M, KwonS, LimJ, KimY et al. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617
BankevichA, NurkS, AntipovD, GurevichAA, DvorkinM et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Com Biol2012; 19:455–477
RichterM, Rosselló-MóraR. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A2009; 106:19126–19131 [View Article]
RichterM, Rosselló-MóraR, GlöcknerFO, PepliesJ. Jspeciesws: A web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics2016; 32:929–931 [View Article]
ChunJ, OrenA, VentosaA, ChristensenH, ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2018; 68:461–466 [View Article] [PubMed]
WellingtonEMH, StackebrandtE, SandersD, WolstrupJ, JorgensenNOG. Taxonomic status of Kitasatosporia, and proposed unification with Streptomyces on the basis of phenotypic and 16s rRNA analysis and emendation of Streptomyces Waksman and Henrici 1943, 339AL. Int J Syst Bacteriol1992; 42:156–160 [View Article]
WayneLG, BrennerDJ, ColwellRR, GrimontPAD, KandlerO et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol1987; 37:463–464