1887

Abstract

An actinobacterium, strain CH5-8, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of spp The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus were observed for strain CH5-8, e.g. -diaminopimelic acid in cell peptidoglycan, MK-9(H), MK-9(H), and MK-9(H) as major menaquinones and anteiso-C, iso-C, and anteiso-C as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus . The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8 showed the highest 16S rRNA gene sequence values to NBRC 12763 (98.9 %), followed by RCU-197 (98.9 %). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8 and its closest relatives, CECT 3313 and RCU-197, were ≤91.6 % and ≤47.4 %, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8 represents a novel species, for which the name sp. nov. is proposed. The type strain is CH5-8 (=TBRC 9950=NBRC 113997).

Funding
This study was supported by the:
  • King Mongkut’s Institute of Technology Ladkrabang Research Fund (Award RE-KRIS/005/64)
    • Principle Award Recipient: ChittiThawai
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2021-07-01
2021-07-29
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