1887

Abstract

A coccoid-shaped, strictly anaerobic, hyperthermophilic and piezophilic organoheterotrophic archaeon, strain Iri35c, was isolated from a hydrothermal chimney rock sample collected at a depth of 2300 m at the Mid-Atlantic Ridge (Rainbow vent field). Cells of strain Iri35c grew at NaCl concentrations ranging from 1–5 % (w/v) (optimum 2.0 %), from pH 5.0 to 9.0 (optimum 7.0–7.5), at temperatures between 50 and 90 °C (optimum 75–80 °C) and at pressures from 0.1 to at least 50 MPa (optimum: 10–30 MPa). The novel isolate grew on complex organic substrates, such as yeast extract, tryptone, peptone or beef extract, preferentially in the presence of elemental sulphur or -cystine; however, these molecules were not necessary for growth. Its genomic DNA G+C content was 54.63 mol%. The genome has been annotated and the metabolic predictions are in accordance with the metabolic characteristics of the strain and of in general. Phylogenetic analyses based on 16S rRNA gene sequences and concatenated ribosomal protein sequences showed that strain Iri35c belongs to the genus and is closer to the species and . Average nucleotide identity scores and DNA–DNA hybridization values between the genome of strain Iri35c and the genomes of the type species of the genus were below the species delineation threshold. Therefore, and considering the phenotypic data presented, strain Iri35c is suggested to represent a novel species, for which the name sp. nov. is proposed, with the type strain Iri35c (=UBOCC M-2026=DSM 111003).

Funding
This study was supported by the:
  • Sino-French IRP 1211 MicrobSea
    • Principle Award Recipient: KarineAlain
  • LabexMER Axis 3 programs ENDIV and CULTIVENT (Award ANR-10-LABX-1)
    • Principle Award Recipient: KarineAlain
  • Grant from the French Ministry of Higher Education and Research
    • Principle Award Recipient: CourtineDamien
  • Program MERLIN Abyss
    • Principle Award Recipient: AlainKarine
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2021-07-08
2021-07-29
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