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Volume 71, Issue 7

sp. nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory No Access

Abstract

A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China. The growth range of NaCl concentration was 0–6.0 % (w/v), with an optimum at 3.0 % (w/v). The temperature range for growth was 10–42 °C, with an optimum at 37 °C. The pH range for growth was pH 7.0–10.0, with an optimum at pH 9.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6 belonged to the genus and showed the highest sequence similarity of 97.8 % to dk3136. The average nucleotide identity and DNA–DNA hybridization values between strain Y6 and the reference strains were 76.9–81.2 % and 20.6–23.6 %, respectively. Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H) and the predominant cellular fatty acids were iso-C, 10-methyl-C and C 9. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids. The peptidoglycan was -2,6-diaminopimelic acid. On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is Y6 (=KCTC 49504=MCCC 1K04765).

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Keyword(s): fisheries processing factory , Nocardioides , Nocardioides malaquae and sewage sludge
Funding
This study was supported by the:
  • Science and Technology Project of Zhoushan city (Award 2017C12032)
    • Principle Award Recipient: GangZheng
  • Zhejiang Provincial Key R & D Program (Award 2021C02024)
    • Principle Award Recipient: GangZheng
  • China Postdoctoral Science Foundation (Award 2018M642382)
    • Principle Award Recipient: CongSun
  • Scientific Research Fund of Oceanography (Award SOA (JB1805))
    • Principle Award Recipient: CongSun
  • Sea Mountain Environmental Management Plan and APEI Construction Project Research (Award DY135-E2-2-05)
    • Principle Award Recipient: MinWu
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: MinWu
© 2021 The Authors
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2021-07-09
2024-04-19
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Nocardioides malaquae sp. nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory
Int J Syst Evol Microbiol 71, 004852 (2021); https://doi.org/10.1099/ijsem.0.004852
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