A novel actinomycete, designated strain KK5PA1T, was isolated from a soil sample collected from Kuan Kreng peat swamp forest, Nakhon Si Thammarat Province, Thailand. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces. Strain KK5PA1T was most closely related to Streptomyces bryophytorum NEAU-HZ10T (98.0 %) and Streptomyces guanduensis 701T (97.6 %). The G+C content of the genomic DNA was 72.3 mol%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain KK5PA1T and those of S. bryophytorum DSM 42138T(25.1 and 79.1 %) and S. guanduensis DSM 41944T(25.1 and 79.7%) were below the thresholds of 70 and 96 % for prokaryotic conspecific assignation. Chemotaxonomic data revealed that strain KK5PA1T possessed MK-9(H6) and MK-9(H8) as the predominant menaquinones. It contained ll-diaminopimelic acid as the diagnostic diamino acid and galactose, glucose, mannose and ribose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, two unidentified aminolipids, five unidentified phospholipids and an unidentified lipid. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and C16 : 0. On the basis of these genotypic and phenotypic data, it is proposed that strain KK5PA1T represents a novel species of the genus Streptomyces, for which the name Streptomyces acididurans sp. nov. is proposed. The type strain is strain KK5PA1T (=TBRC 13094T=NBRC 114802T).
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