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Abstract

Strain MaA-C15, a Gram-stain-negative, non-spore-forming and strictly aerobic bacterium, was isolated from a xenic culture of in the Republic of Korea. Cells were motile rods showing positive reactions in catalase and oxidase tests. Growth was observed between 15 and 37 °C (optimum, 30 °C), between pH 6.0 and pH 11.0 (optimum, pH 7.5) and in the presence of 0–2.0 % (w/v) NaCl (optimum, 0 %). Strain MaA-C15 contained C, 11-methyl-C 7, cyclo-C 8 and summed feature 8 (C 6 and/or C 7) as the major cellular fatty acids and ubiquinone-10 as the sole respiratory quinone. Phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid and three unidentified phospholipids were detected as the major polar lipids. The G+C content of the genomic DNA was 64.1 mol%. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and genome sequences revealed that strain MaA-C15 formed a phyletic lineage with YIM M12096 within the family . Strain MaA-C15 was most closely related to DSM 21822 with a 98.2 % 16S rRNA sequence similarity. Average nucleotide identity and DNA–DNA hybridization values between strain MaA-C15 and DSM 21822 were 75.4 and 20.1 %, respectively. Based on the results of phenotypic, chemotaxonomic and molecular analyses, strain MaA-C15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MaA-C15 (=KACC 21226=JCM 33503).

Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202029201)
    • Principle Award Recipient: CheOk Jeon
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2021-07-02
2021-07-29
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