1887

Abstract

A novel bacterial strain, named HC41, was isolated from a cyanobacterial bloom sample and was characterized as Gram-stain-negative, rod-shaped and non-motile. According to 16S rRNA phylogenetic analyses, this strain HC41 belongs to the family and is most closely related to KACC 17062 (=MIC 2059; 98.63 %) and 5YN10-9 (=KACC 11603; 93.64 %). The genome size and DNA G+C content of strain HC41 were 4.8 Mbp and 64.17 mol%, respectively. Moreover, the average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain HC41 and KACC 17062 were 81.8, 43.1 and 90.89 %, respectively. Additionally, strain HC41 exhibited weak catalase and oxidase activities and had no motility (swimming and swarming motilities). The cells grew at 11–40 °C (optimum, 30 °C), pH 5.5–8.0 (optimum, pH 7) and with 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl) in Reasoner’s 2A medium. Its major respiratory quinone was ubiquinone-8 and its major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Furthermore, C and summed feature 3 (C ω7 and/or C ω6c; C ω6 and/or C ω7) were the predominant cellular fatty acids in strain HC41 according to fatty acid methyl ester analysis. Based on its genotypic and phenotypic characteristics, strain HC41 was identified as representing a novel species, for which the name sp. nov. is proposed (=KACC 22091=DSM 111425).

Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202002108)
    • Principle Award Recipient: WoojunPark
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004845
2021-07-07
2021-07-29
Loading full text...

Full text loading...

References

  1. Pfennig N. Rhodocyclus purpureus gen. nov. and sp. nov. a ring-shaped, vitamin-B12-requiring member of family Rhodospirillaceae. Int J Syst Bacteriol 1978; 28:283–288 [View Article]
    [Google Scholar]
  2. Reinhold-Hurek B, Hurek T. Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov. Int J Syst Evol Microbiol 2000; 50:649–659 [View Article]
    [Google Scholar]
  3. Thrash JC, Pollock J, Torok T, Coates JD. Description of the novel perchlorate-reducing bacteria Dechlorobacter hydrogenophilus gen. nov., sp. nov. and Propionivibrio militaris, sp. nov. Appl Microbiol Biotechnol 2010; 86:335–343 [View Article] [PubMed]
    [Google Scholar]
  4. Achenbach LA, Michaelidou U, Bruce RA, Fryman J, Coates JD. Dechloromonas agitata gen. nov., sp. nov. and Dechlorosoma suillum gen. nov., sp. nov., two novel environmentally dominant (per)chlorate-reducing bacteria and their phylogenetic position. Int J Syst Evol Microbiol 2001; 51:527–533 [View Article] [PubMed]
    [Google Scholar]
  5. Lew B, Stief P, Beliavski M. Characterization of denitrifying granular sludge with and without the addition of external carbon source. Bioresour Technol 2012; 124:413–420 [View Article] [PubMed]
    [Google Scholar]
  6. Chun J, Kang JY, Jung YC, Jahng KY. Niveibacterium umoris gen. nov., sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 2016; 66:997–1002 [View Article]
    [Google Scholar]
  7. Liu C-T, Lin S-Y, Hameed A, Liu Y-C, Hsu Y-H et al. Oryzomicrobium terrae gen. nov., sp. nov., of the family Rhodocyclaceae isolated from paddy soil. Int J Syst Evol Microbiol 2017; 67:183–189 [View Article] [PubMed]
    [Google Scholar]
  8. Meijer WG, Nienhuis-Kuiper ME, Hansen TA. Fermentative bacteria from estuarine mud: phylogenetic position of Acidaminobacter hydrogenoformans and description of a new type of gram-negative, propionigenic bacterium as Propionibacter pelophilus gen. nov., sp. nov. Int J Syst Bacteriol 1999; 49:1039–1044 [View Article]
    [Google Scholar]
  9. Tanaka K, Nakamura K, Mikami E. Fermentation of maleate by a gram-negative strictly anaerobic non-spore former, Propionivibrio dicarboxylicus gen. nov., sp. nov. Arch Microbiol 1990; 154:323–328 [View Article]
    [Google Scholar]
  10. Corteselli EM, Aitken MD, Singleton DR. Rugosibacter aromaticivorans gen. nov., sp. nov., a bacterium within the family Rhodocyclaceae, isolated from contaminated soil, capable of degrading aromatic compounds. Int J Syst Evol Microbiol 2017; 67:311–318 [View Article] [PubMed]
    [Google Scholar]
  11. Kang JY, Chun J, Jung YC, Jahng KY. Viridibacterium curvum gen. nov., sp. nov., isolated from freshwater. J Microbiol 2017; 55:514–519 [View Article]
    [Google Scholar]
  12. Weon H-Y, Kim B-Y, Yoo S-H, Kwon S-W, Go S-J et al. Uliginosibacterium gangwonense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea. Int J Syst Evol Microbiol 2008; 58:131–135 [View Article]
    [Google Scholar]
  13. Hwang WM, Kim SM, Kang K, Ahn TY. Uliginosibacterium sediminicola sp. nov., isolated from freshwater sediment. Int J Syst Evol Microbiol 2018; 68:924–929 [View Article] [PubMed]
    [Google Scholar]
  14. Park C, Kim M, Lee B-H, Lee K-E, Park W. Sphingomonas changnyeongensis sp. nov., isolated from the Hapcheon-Changnyeong barrage area in the Nakdong river. Int J Syst Evol Microbiol 2020; 70:6091–6097 [View Article] [PubMed]
    [Google Scholar]
  15. Kim M, Lee J, Yang D, Park HY, Park W. Seasonal dynamics of the bacterial communities associated with cyanobacterial blooms in the Han River. Environ Pollut 2020; 266:115198 [View Article] [PubMed]
    [Google Scholar]
  16. Shin Y, Lee B-H, Lee K-E, Park W. Pseudarthrobacter psychrotolerans sp. nov., a cold-adapted bacterium isolated from Antarctic soil. Int J Syst Evol Microbiol 2020; 70:6106–6114 [View Article] [PubMed]
    [Google Scholar]
  17. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article] [PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article] [PubMed]
    [Google Scholar]
  19. Wagner J, Coupland P, Browne HP, Lawley TD, Francis SC et al. Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification. BMC Microbiol 2016; 16:274 [View Article] [PubMed]
    [Google Scholar]
  20. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 2013; 10:563–569 [View Article] [PubMed]
    [Google Scholar]
  21. Richter M, Rosselló-Móra R Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article]
    [Google Scholar]
  22. Luo C, Rodriguez-R LM, Konstantinidis KT. MyTaxa: an advanced taxonomic classifier for genomic and metagenomic sequences. Nucleic Acids Res 2014; 42:e73 [View Article] [PubMed]
    [Google Scholar]
  23. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  24. Konstantinidis KT, Tiedje JM. Towards a genome-based taxonomy for prokaryotes. J Bacteriol 2005; 187:6258–6264 [View Article] [PubMed]
    [Google Scholar]
  25. Park C, Lee YS, Park S-Y, Park W. Methylobacterium currus sp. nov., isolated from a car air conditioning system. Int J Syst Evol Microbiol 2018; 68:3621–3626 [View Article] [PubMed]
    [Google Scholar]
  26. Smibert RM, Krieg NR. Methods for general and molecular bacteriology. American Society for Microbiology 1994607–654 [View Article]
    [Google Scholar]
  27. Sasser M. Bacterial Identification by Gas Chromatographic Analysis of Fatty Acids Methyl Esters (GC-FAME), Midi Tech Note. pp 457–469
    [Google Scholar]
  28. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  29. Jung J, Park W. Pedobacter jeongneungensis sp. nov., isolated from forest soil. J Microbiol 2012; 50:660–664 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004845
Loading
/content/journal/ijsem/10.1099/ijsem.0.004845
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error