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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 7

sp. nov., isolated from a cyanobacterial bloom sample Free

Abstract

A novel bacterial strain, named HC41, was isolated from a cyanobacterial bloom sample and was characterized as Gram-stain-negative, rod-shaped and non-motile. According to 16S rRNA phylogenetic analyses, this strain HC41 belongs to the family and is most closely related to KACC 17062 (=MIC 2059; 98.63 %) and 5YN10-9 (=KACC 11603; 93.64 %). The genome size and DNA G+C content of strain HC41 were 4.8 Mbp and 64.17 mol%, respectively. Moreover, the average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain HC41 and KACC 17062 were 81.8, 43.1 and 90.89 %, respectively. Additionally, strain HC41 exhibited weak catalase and oxidase activities and had no motility (swimming and swarming motilities). The cells grew at 11–40 °C (optimum, 30 °C), pH 5.5–8.0 (optimum, pH 7) and with 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl) in Reasoner’s 2A medium. Its major respiratory quinone was ubiquinone-8 and its major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Furthermore, C and summed feature 3 (C ω7 and/or C ω6c; C ω6 and/or C ω7) were the predominant cellular fatty acids in strain HC41 according to fatty acid methyl ester analysis. Based on its genotypic and phenotypic characteristics, strain HC41 was identified as representing a novel species, for which the name sp. nov. is proposed (=KACC 22091=DSM 111425).

  • Received:
  • Accepted:
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Keyword(s): cyanobacterial bloom , freshwater , genomics , Microcystis aeruginosa , Niveibacterium and Proteobacteria
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202002108)
    • Principle Award Recipient: WoojunPark
© 2021 The Authors
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2021-07-07
2025-01-18
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Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample
Int J Syst Evol Microbiol 71, 004845 (2021); https://doi.org/10.1099/ijsem.0.004845
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