RT Journal Article SR Electronic(1) A1 Ming, Hong A1 Cheng, Li-jiao A1 Yi, Bing-fang A1 Xia, Ting-ting A1 Niu, Ming-ming A1 Zhao, Zi-yu A1 Liu, Bing-bing A1 Nie, Guo-xing A1 Cui, Cai-xiaYR 2021 T1 Brachybacterium subflavum sp. nov., a novel actinobacterium isolated from the foregut of grass carp JF International Journal of Systematic and Evolutionary Microbiology, VO 71 IS 6 OP SP 004839 DO https://doi.org/10.1099/ijsem.0.004839 PB Microbiology Society, SN 1466-5034, AB A novel actinobacterium, designated CFH 10395T, was isolated from the foregut of grass carp (Ctenopharyngodon idella), which had been fed with ginseng extract supplement. The taxonomic position was investigated by a polyphasic approach. Cells of CFH 10395T were Gram-staining-positive, aerobic, ovoid-shaped, non-spore-forming and non-motile. On the basis of the results of 16S rRNA gene sequence analysis, CFH 10395T was most closely related to Brachybacterium endophyticum KCTC 49087T, Brachybacterium squillarum JCM 16464T and Brachybacterium paraconglomeratum JCM 17781T (97.85%, 97.51 and 97.29% similarity, respectively). CFH 10395T grew at 4–37 °C, pH 5.0–9.0 and in the presence of up to 10.0 % NaCl (w/v). The dominant menaquinone was MK-7. The whole-cell sugars were rhamnose, glucose, mannose and galactose. meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The genome size was 3.99 Mbp with a DNA G+C content of 71.9 mol%. On the basis of the results of phylogenetic analysis, physiological properties, chemotaxonomic characteristics, low average nucleotide identity (ANI) and digital DDH (dDDH) results [ANI calculated using MUMmer (ANIm) <87 %, ANI calculated using blast (ANIb) <83 % and dDDH <23 %], it is concluded that CFH 10395T represents a novel species of the genus Brachybacterium , for which the name Brachybacterium subflavum sp. nov., is proposed. The type strain is CFH 10395T (=CGMCC 1.13804T=KCTC 49235T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004839