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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 6

sp. nov., a novel actinobacterium isolated from the foregut of grass carp Free

Abstract

A novel actinobacterium, designated CFH 10395, was isolated from the foregut of grass carp (), which had been fed with ginseng extract supplement. The taxonomic position was investigated by a polyphasic approach. Cells of CFH 10395 were Gram-staining-positive, aerobic, ovoid-shaped, non-spore-forming and non-motile. On the basis of the results of 16S rRNA gene sequence analysis, CFH 10395 was most closely related to KCTC 49087, JCM 16464 and JCM 17781 (97.85%, 97.51 and 97.29% similarity, respectively). CFH 10395 grew at 4–37 °C, pH 5.0–9.0 and in the presence of up to 10.0 % NaCl (w/v). The dominant menaquinone was MK-7. The whole-cell sugars were rhamnose, glucose, mannose and galactose. -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major fatty acids were anteiso-C, anteiso-C and iso-C. The genome size was 3.99 Mbp with a DNA G+C content of 71.9 mol%. On the basis of the results of phylogenetic analysis, physiological properties, chemotaxonomic characteristics, low average nucleotide identity (ANI) and digital DDH (dDDH) results [ANI calculated using MUMmer (ANIm) <87 %, ANI calculated using (ANIb) <83 % and dDDH <23 %], it is concluded that CFH 10395 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is CFH 10395 (=CGMCC 1.13804=KCTC 49235).

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  • Accepted:
  • Published Online:
Keyword(s): Brachybacterium subflavum sp. nov. , grass carp and polyphasic taxonomy
Funding
This study was supported by the:
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: HongMing
  • Key Technologies R&D Program of Henan Province (Award 202102110107)
    • Principle Award Recipient: HongMing
  • Innovation Scientists and Technicians Troop Construction Projects of Henan Province (Award CXTD2016043)
    • Principle Award Recipient: Guo-XingNie
  • National Natural Science Foundation of China (Award 31800001)
    • Principle Award Recipient: Bing-bingLiu
  • Key Scientific Research Project of Colleges and Universities in Henan Province (Award 20B530005)
    • Principle Award Recipient: Cai-xiaCui
© 2021 The Authors
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2025-03-23
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Brachybacterium subflavum sp. nov., a novel actinobacterium isolated from the foregut of grass carp
Int J Syst Evol Microbiol 71, 004839 (2021); https://doi.org/10.1099/ijsem.0.004839
/content/journal/ijsem/10.1099/ijsem.0.004839
/content/journal/ijsem/10.1099/ijsem.0.004839
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