1887

Abstract

Two novel Gram-staining-negative, aerobic, cocci-shaped, non-motile, non-spore forming, pink-pigmented bacteria designated strains T6 and T18, were isolated from a biocrust (biological soil crust) sample from the vicinity of the Tabernas Desert (Spain). Both strains were catalase-positive and oxidase-negative, and grew under mesophilic, neutrophilic and non-halophilic conditions. According to the 16S rRNA gene sequences, strains T6 and T18 showed similarities with CGMCC 1.10758 and CP2C (98.11 and 98.55% gene sequence similarity, respectively). The DNA G+C content was 69.80 and 68.96% for strains T6 and T18, respectively; the average nucleotide identity by (ANIb) and digital DNA–DNA hybridization (dDDH) values confirmed their adscription to two novel species within the genus . The predominant fatty acids were summed feature 8 (Cω7Cω6), C, C 2-OH and summed feature 3 (Cω7Cω6). According to he results of the polyphasic study, strains T6 and T18 represent two novel species in the genus (which currently includes only three species), for which names sp. nov. (type strain T6 = CECT 30228=DSM 112073) and sp. nov. (type strain T18=CECT 30229=DSM 112074) are proposed, respectively.

Funding
This study was supported by the:
  • Ministerio de Ciencia, Innovación y Universidades (Award FPU18/02578)
    • Principle Award Recipient: ÀngelaVidal-Verdú
  • Ministerio de Ciencia, Innovación y Universidades (Award FPU17/04184)
    • Principle Award Recipient: EstherMolina-Menor
  • Ministerio de Ciencia, Innovación y Universidades (Award RTI2018-095584-B-C41-42-43-44)
    • Principle Award Recipient: ApplicableNot
  • European CSA on biological standardization, BioRoboost (Award 820699)
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2021-07-22
2021-07-29
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