1887

Abstract

An endophytic actinobacterium, designated strain CA1R205, was isolated from the surface-sterilized root of L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish white aerial mycelium. The spiral spore chains were produced directly on aerial mycelium. CA1R205 was found to have -diaminopimelic acid in the cell peptidoglycan, galactose, glucose, mannose and ribose as whole-cell reducing sugars, MK-10(H), MK-9(H), MK-10(H), MK-9(H), MK-10(H) and MK-10(H) as menaquinones and iso-C, anteiso-C, iso-C and C as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were detected in the cells. These characteristics were consistent the typical chemotaxonomic properties of members the genus . The taxonomic affiliation at the genus level of this strain could be confirmed using its 16S rRNA gene sequence data. CA1R205 showed the highest 16S rRNA gene sequence similarity value to NRRL B-5491 (98.9 %), followed by HM 35 (98.8 %). Digital DNA–DNA hybridization and average nucleotide identity-by (ANIb) values between CA1R205 and NRRL B-5491 were 27.2 and 81.5 %, respectively. The DNA G+C content of genomic DNA was 70.7 mol%. Due to the differences in physiological, biochemical and genotypic data, CA1R205 could be discriminated from its closest neighbour. Thus, CA1R205 should be recognized as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CA1R205 (=TBRC 11244=NBRC 114295).

Funding
This study was supported by the:
  • Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang (Award RA/TA-2562-M-021)
    • Principle Award Recipient: AchararakNammali
  • King Mongkut’s Institute of Technology Ladkrabang Research Fund (Award KREF046301)
    • Principle Award Recipient: ChittiThawai
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