1887

Abstract

A novel strain of a member of the genus strain PS-1, was isolated from the skin of fresh water pufferfish () collected from Mahanadi River, India. Cells were Gram-stain-negative, aerobic, coccoid and non-motile. The predominant polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phospholipid (PL) and the cell wall sugars were glucose, galactose and ribose. The major cellular fatty acids of PS-1 were Cω9 (30.67 %), Cω7 (19.54 %), C (15.87 %), C (7.35 %) and C 3-OH (6.77 %). The genome size was 3.5 Mbp and the DNA G+C content was 41.97 %. Gene ontology study revealed that the major fraction of genes were associated with biological processes (53.99 %) followed by molecular function (30.42 %) and cellular components (15.58 %). Comparisons of 16S rRNA gene sequences revealed 97.94–97.05 % sequence similarity with the closely related type strains of species of the genus . The average nucleotide identity (ANI) and average amino acid identity (AAI) of PS-1 with reference strains of species of the genus with validly published names were bellow 95–96 and the corresponding DNA–DNA hybridization (DDH) values were below 70 %. A phylogenomic tree based on core genome analysis supported these results. Genotypic and phenotypic characteristics of PS-1 indicate that the strain represents a novel species of the genus and the name sp. nov. is proposed. The type strain is PS-1 (=JCM 34131=NCIMB 15260)

Funding
This study was supported by the:
  • Department of Biotechnology, New Delhi (Award D.O.No. BT/PR7661/AAQ/3/629/2013)
    • Principle Award Recipient: SubrataK Das
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2021-06-15
2021-07-29
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