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Abstract

A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621, was isolated from rhizosphere soil of in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach. GY 10621 was positive for catalase and oxidase. Growth occurred at 4–42 °C (optimum 30–37 °C), at pH 5.0–9.0 (optimum pH 7.0) and in the presence of 0–5% NaCl (w/v) (optimum 1–3%). The main menaquinones were MK-9 (H) (92.2 %) and MK-10 (7.8 %). The major cellular fatty acids were anteiso-C and C. The peptidoglycan was the type A4α (-Lys-Ser--Glu). The polar lipids included four phosphoglycolipids, four glycolipids, an unidentified lipid and six unidentified phospholipids. The DNA G+C content of the type strain was 71.7 mol%. On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621 was KCTC 13155 (97.16 %), followed by NBRC 16159 (96.39 %). The calculated results indicated that compared with GY 10621, the average nucleotide identity (ANI) values of three strains closely related to GY 10621 (the two aforementioned type strains and ‘’ Marseille-P3815) were 74.18–94.97 %, and the digital DNA–DNA hybridization (dDDH) values were 20.3–60.6 %. The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621 should be assigned to the genus . On the basis of evidence from polyphasic studies, GY 10621 should be designated as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GY 10621 (=CGMCC 1.17411=KCTC 49515).

Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 32060001)
    • Principle Award Recipient: YiJiang
  • the National Natural Science Foundation of China (Award 81960164)
    • Principle Award Recipient: LifangYang
  • the Specific research project of Guangxi for research bases and talents (Award AD18281066)
    • Principle Award Recipient: MingguoJiang
  • the Science and Technology Major Project of Guangxi (Award AA18242026)
    • Principle Award Recipient: MingguoJiang
  • the National Natural Science Foundation of China (Award 31660005)
    • Principle Award Recipient: MingguoJiang
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