1887

Abstract

Four novel independent strains of spp. were isolated from faeces of alpaca (SL1232), cattle (KCJ4950), and from respiratory tract of wild California sea lions (CSL7508, CSL7591). The strains were indole-, oxidase- and catalase-negative, non-spore-forming, non-motile Gram-positive cocci in short and long chains, facultative anaerobes. The 16S rRNA gene of SL1232 and KCJ4950 shared 99.40–99.60% nucleotide similarity to strains of , and the 16S rRNA gene of CSL7508 and CSL7591 demonstrated 98.72 and 98.92% similarity, respectively, to . All other known species had the 16S rRNA gene sequence similarities of ≤95%. The genomes were sequenced for the novel strains. Average nucleotide identity (ANI) analysis for strains SL1232 and KCJ4950, showed the highest similarity to and with 85.21, 87.17, 88.47, 85.54, 87.47 and 88.89%, respectively, and strains CSL7508 and CSL7591 to with 87.16 and 83.97%, respectively. Results of ANI were confirmed by pairwise digital DNA–DNA hybridization and phylogeny, which also revealed that the strains belong to three novel species of the genus . Phenotypical features of the novel species were in congruence with closely related members of the genus and gave negative reactions with the tested Lancefield serological groups (A-D, F and G). MALDI-TOF mass spectrometry supported identification of the species. Based on these data, we propose three novel species of the genus , for which the name sp. nov. is proposed with the type strain SL1232 (=NCTC 14341=DSM 110741=CCUG 74371), sp. nov. is proposed with the type strain CSL7508 (=NCTC 14410=DSM 110742=CCUG 74374) and sp. nov. is proposed with the type strain CSL7591 (=NCTC 14455=DSM 111148=CCUG 74655). The genome G+C content is 36.89, 34.85, and 35.34 % and draft genome sizes are 1906993, 1581094 and 1656080 bp for strains SL1232, CSL7508, and CSL7591, respectively.

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2021-05-17
2022-01-24
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