1887

Abstract

A novel Gram-stain-negative, rod-shaped, non-motile, yellowish bacterium, designated strain 1.3611, was isolated from the wormcast of . The strain grew optimally at 30–37 ℃, at pH 7.0 and with 0–1.0 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, strain 1.3611 showed the highest degree of 16S rRNA gene sequence similarity to HAL-9 (97.0 %), followed by Y3L14 (95.8 %). The respiratory quinone of strain 1.3611 was menaquinone-7 (MK-7) and its major cellular fatty acids were iso-C (41.3 %), summed feature 3 (C 7 and/or C 6, 22.1 %) and iso-C 3-OH (16.2 %). The major polar lipids were sphingophospholipid, phosphatidylethanolamine, four unidentified glycolipids, two unidentified phospholipids and five unidentified polar lipids. The genomic DNA G+C content was 39.0 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between the genomes of strain 1.3611 and HAL-9 were 37.9 and 88.9 %, respectively. According to the phenotypic and chemotaxonomic phylogenetic results, strain 1.3611 should represent a novel species of the genus , for which the name sp. nov. is proposed, with strain 1.3611 (=KCTC 62980=CCTCC AB 2018349) as the type strain.

Funding
This study was supported by the:
  • the Ten-thousand Talents Program in Yunnan Province (Award YNWR-CYJS-2019-042)
    • Principle Award Recipient: MingHe Mo
  • the Department of Science and Technology of Yunnan Province (Award 202001BB050072)
    • Principle Award Recipient: MingHe Mo
  • the Department of Science and Technology of Yunnan Province (Award 2019ZG00901)
    • Principle Award Recipient: MingHe Mo
  • the Program of Guizhou Provincial Tobacco Company (Award 2021XM12)
    • Principle Award Recipient: MingHe Mo
  • the National Natural Science Foundation Program of China (Award 31660544)
    • Principle Award Recipient: MingHe Mo
  • the National Natural Science Foundation Program of China (Award 31870091)
    • Principle Award Recipient: MingHe Mo
  • the National Natural Science Foundation Program of China (Award 31960022)
    • Principle Award Recipient: MingHe Mo
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2021-05-28
2024-03-29
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