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Abstract

Members within the family are morphologically and genetically highly diverse, and originate mostly from coastal marine environments. In this study, a novel species of this family, designated M0103, was isolated from the surface of a sea snail . Strain M0103 is Gram-stain-negative, halophilic, non-motile and non-Bacteriochlorophyll -producing bacterium. Several phenotypic characteristics of the isolate were similar to other species within this family, such as the sole respiratory quinone Q-10 and major fatty acid components C 7c, C and C. Strain M0103 contains a diphosphatidylglycerol, a phosphatidylglycerol, a phosphatidylcholine, a phosphatidy ethanolamine, a phosphatidylinositol, five unidentified phospholipids and four unidentified polar lipids. Based on the 16S rRNA gene sequence analysis, this isolate showed the closest phylogenetic relationship with ‘’ GH1-23 (95.1 %). Values of average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) of genome sequences were of 70.1–76.4 % and 18.3–20.9 % between the isolate and 24 closely related type strains. Analysis the 4.0 Mb genome of strain M0103 revealed several putative genes associated with cellular stress resistance, which may play protective roles for the isolate in the adaptation to a marine environment. Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain M0103 represents a novel genus and novel species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is M0103 (=MCCC 1K03619=KCTC 62358).

Funding
This study was supported by the:
  • The Special Project for the Base of Guangxi Science and Technology and Talents (Award AD17129019)
    • Principle Award Recipient: ShushiHuang
  • Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry (Award 17-259-74)
    • Principle Award Recipient: ShushiHuang
  • The Fundamental Research Funds for Guangxi Academy of Sciences (Award 2018YBJ303)
    • Principle Award Recipient: WenjinHu
  • The Fundamental Research Funds for Guangxi Academy of Sciences (Award 2019YBJ101)
    • Principle Award Recipient: XinliPan
  • National Natural Science Foundation of China (Award 31560017)
    • Principle Award Recipient: ShushiHuang
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2021-06-28
2024-03-29
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