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Abstract

Strain HNM0947, representing a novel actinobacterium, was isolated from the coral collected from the coast of Wenchang, Hainan, China. The strain was found to have morphological and chemotaxonomic characteristics consistent with the genus . The organism formed abundant fragmented substrate mycelia and aerial mycelia which differentiated into non-motile, rod-shaped spores. Whole-cell hydrolysates contained -diaminopimelic acid and no diagnostic sugars. The major menaquinones were MK-10(H), MK-10(H) and MK-10(H). The major phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were -C, -C, C, C 10-methyl (TBSA) and -C. The G+C content was 71.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HNM0947 belonged to the genus and shared highest sequence similarity to YIM 90010 (98.8%), YIM 90004(98.5%) and DSM 44524 (98.3%). The strain HNM0947 was distinguished from its closest type strain by low average nucleotide identity (90.8%) and dDDH values (60.4%) respectively. Based on genotypic, chemotaxonomic and phenotypic characteristics, it was concluded that strain HNM0947 represents a novel species of the genus whose name was proposed as sp. nov. The type strain was HNM0947 (=CCTCC AA 2020015 =KCTC 49525 ).

Funding
This study was supported by the:
  • High-level talent grant of basic and applied basic research project (natural science) of Hainan province in 2019 (Award 2019RC133)
    • Principle Award Recipient: ShuangqingZhou
  • High-level talent grant of basic and applied basic research project (natural science) of Hainan province in 2019 (Award 2019RC069)
    • Principle Award Recipient: XiaolongHuang
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2021-06-15
2021-07-29
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