1887

Abstract

Two Gram-stain-negative and non-flagellated bacteria, YSTF-M3 and YSTF-M6, were isolated from a tidal flat from Yellow Sea, Republic of Korea, and subjected to a polyphasic taxonomic study. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strains YSTF-M3 and YSTF-M6 belong to the genera and of the family , respectively. The 16S rRNA gene sequence similarities between strain YSTF-M3 and the type strains of species and between strain YSTF-M6 and the type strains of species were 94.1–98.4 and 97.3–98.3 %, respectively. The ANI and dDDH values between genomic sequences of strain YSTF-M3 and the type strains of five species and between those of strain YSTF-M6 and the type strains of three species were in ranges of 77.0–83.2 and 20.7–27.1 % and 79.4–81.5 and 22.3–23.9 %, respectively. The DNA G+C contents of strain YSTF-M3 and YSTF-M6 from genomic sequences were 34.1 and 31.1 %, respectively. Both strains contained MK-6 as predominant menaquinone and phosphatidylethanolamine as only major phospholipid identified. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strains YSTF-M3 and YSTF-M6 are separated from recognized species of the genera and , respectively. On the basis of the data presented, strains YSTF-M3 (=KACC 21639=NBRC 114499) and YSTF-M6 (=KACC 21640=NBRC 114500) are considered to represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed.

Funding
This study was supported by the:
  • Rural Development Administration (Award PJ014442)
    • Principle Award Recipient: SooyeonPark
  • National Institute of Biological Resources (Award project on survey of indigenous species of Korea)
    • Principle Award Recipient: Jung-HoonYoon
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2021-06-04
2021-06-19
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