%0 Journal Article %A Liu, Guan %A Yu, Xia %A Luo, Jingjing %A Hu, Yanjie %A Dong, Lingling %A Jiang, Guanglu %A Huo, Fengmin %A Wang, Fen %A Liang, Qian %A Huang, Hairong %T Mycobacterium vicinigordonae sp. nov., a slow-growing scotochromogenic species isolated from sputum %D 2021 %J International Journal of Systematic and Evolutionary Microbiology, %V 71 %N 5 %@ 1466-5034 %C 004796 %R https://doi.org/10.1099/ijsem.0.004796 %K Mycobacterium vicinigordonae %K strain 24T %K non-tuberculous mycobacteria %K taxonomy %I Microbiology Society, %X A slow-growing, scotochromogenic mycobacterial strain (24T) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24T to the Mycobacterium gordonae complex, which includes Mycobacterium gordonae and Mycobacterium paragordonae . The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based on hsp65 and rpoB sequences strongly supported the taxonomic status of strain 24T as a representative of a species distinct from the other members of the M. gordonae complex. The genomic G+C content of strain 24T was 65.40mol%. Genomic comparisons showed that strain 24T and M. gordonae ATCC 14470T had an average nucleotide identity (ANI) value of 81.00 % and a DNA–DNA hybridization (DDH) value of 22.80 %, while the ANI and DDH values between strain 24Tand M. paragordonae 49 061T were 80.98 and 22.80 %, respectively. In terms of phylogenetic, phenotypic and chemotaxonomic features, strain 24T is distinguishable from its closest phylogenetic relatives and represents a novel species of the genus Mycobacterium , therefore the name Mycobacterium vicinigordonae sp. nov. is proposed. The type strain is 24T (=CMCC 93559T=DSM 105979T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004796