1887

Abstract

A slow-growing, scotochromogenic mycobacterial strain (24) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24 to the complex, which includes and . The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based on and sequences strongly supported the taxonomic status of strain 24 as a representative of a species distinct from the other members of the complex. The genomic G+C content of strain 24 was 65.40mol%. Genomic comparisons showed that strain 24 and ATCC 14470 had an average nucleotide identity (ANI) value of 81.00 % and a DNA–DNA hybridization (DDH) value of 22.80 %, while the ANI and DDH values between strain 24and 49 061 were 80.98 and 22.80 %, respectively. In terms of phylogenetic, phenotypic and chemotaxonomic features, strain 24 is distinguishable from its closest phylogenetic relatives and represents a novel species of the genus , therefore the name sp. nov. is proposed. The type strain is 24 (=CMCC 93559=DSM 105979).

Funding
This study was supported by the:
  • Chinese Society of Clinical Oncology (CN) (Award WX17Q33)
    • Principle Award Recipient: GuanLiu
  • Natural Science Foundation of Hubei Province (CN) (Award 2017CFB364)
    • Principle Award Recipient: GuanLiu
  • Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (CN) (Award DFL20181602)
    • Principle Award Recipient: HairongHuang
  • Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (Award ZYLX201824)
    • Principle Award Recipient: HairongHuang
  • Municipal Science & Technology commission (Award Z181100001718092)
    • Principle Award Recipient: HairongHuang
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 81672065)
    • Principle Award Recipient: XiaYu
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 81802057)
    • Principle Award Recipient: HairongHuang
  • Infectious Diseases Special Project, Ministry of Health of China (Award 2018ZX10302302-004-005)
    • Principle Award Recipient: XiaYu
  • Infectious Diseases Special Project, Ministry of Health of China (Award 2018ZX10302301-004)
    • Principle Award Recipient: HairongHuang
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2021-05-11
2022-01-24
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