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Abstract
A slow-growing, scotochromogenic mycobacterial strain (24T) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24T to the Mycobacterium gordonae complex, which includes Mycobacterium gordonae and Mycobacterium paragordonae . The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based on hsp65 and rpoB sequences strongly supported the taxonomic status of strain 24T as a representative of a species distinct from the other members of the M. gordonae complex. The genomic G+C content of strain 24T was 65.40mol%. Genomic comparisons showed that strain 24T and M. gordonae ATCC 14470T had an average nucleotide identity (ANI) value of 81.00 % and a DNA–DNA hybridization (DDH) value of 22.80 %, while the ANI and DDH values between strain 24Tand M. paragordonae 49 061T were 80.98 and 22.80 %, respectively. In terms of phylogenetic, phenotypic and chemotaxonomic features, strain 24T is distinguishable from its closest phylogenetic relatives and represents a novel species of the genus Mycobacterium , therefore the name Mycobacterium vicinigordonae sp. nov. is proposed. The type strain is 24T (=CMCC 93559T=DSM 105979T).
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Funding
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Chinese Society of Clinical Oncology (CN)
(Award WX17Q33)
- Principle Award Recipient: GuanLiu
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Natural Science Foundation of Hubei Province (CN)
(Award 2017CFB364)
- Principle Award Recipient: GuanLiu
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Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (CN)
(Award DFL20181602)
- Principle Award Recipient: HairongHuang
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Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support
(Award ZYLX201824)
- Principle Award Recipient: HairongHuang
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Municipal Science & Technology commission
(Award Z181100001718092)
- Principle Award Recipient: HairongHuang
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Innovative Research Group Project of the National Natural Science Foundation of China
(Award 81672065)
- Principle Award Recipient: XiaYu
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Innovative Research Group Project of the National Natural Science Foundation of China
(Award 81802057)
- Principle Award Recipient: HairongHuang
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Infectious Diseases Special Project, Ministry of Health of China
(Award 2018ZX10302302-004-005)
- Principle Award Recipient: XiaYu
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Infectious Diseases Special Project, Ministry of Health of China
(Award 2018ZX10302301-004)
- Principle Award Recipient: HairongHuang