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Volume 71, Issue 4

sp. nov., a novel endophytic actinobacterium isolated from branch of No Access

Abstract

A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2, was isolated from a surface-sterilized branch of collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China. Strain CMS6Z-2 grew at 10–37 °C (optimum, 37 °C), pH 6.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 0–10.0 % (w/v) NaCl (optimum, 0–1.0 %). Strain CMS6Z-2 possessed -diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H) as the predominant menaquinone. The major fatty acids were iso-C, C and C 9. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. The G+C content of the genomic DNA was 74.1 mol%. Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2 should be assigned to the genus and its closest relative was IP6SC6 with 98.3 % similarity. Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2 belonged to the genus and formed a robust cluster with IP6SC6 within the genus . The average nucleotide identity value and estimated digital DNA–DNA hybridization value between strain CMS6Z-2 and the type strain of were 81.5 and 23.9 %, respectively. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CMS6Z-2 (=KCTC 49240=CGMCC4.7549).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CMS6Z-2 , endophytic actinobacterium , Kandelia candel and Phycicoccus flavus
Funding
This study was supported by the:
  • CAMS innovation Fund for Medical Sciences (Award 2017-12M-1-012)
    • Principle Award Recipient: Cheng-HangSun
  • CAMS innovation Fund for Medical Sciences (Award CAMS 2017-12M-B&R-08)
    • Principle Award Recipient: Cheng-HangSun
  • Guizhou Provincial Science and Technology Foundation (Award Qian Ke He Jichu[2019]1347)
    • Principle Award Recipient: TuoLi
  • National Natural Sciences Foundation of China (Award 81960642)
    • Principle Award Recipient: LiTuo
© 2021 The Authors
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2024-04-25
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Phycicoccus flavus sp. nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel
Int J Syst Evol Microbiol 71, 004794 (2021); https://doi.org/10.1099/ijsem.0.004794
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