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Abstract

A Gram-stain-negative, aerobic, flagellated, non-spore-forming, rod-shaped bacterium, named B2R-29, was isolated from water collected from a crater lake on Da Hinggan mountain, PR China. Strain B2R-29 was oxidase- and catalase-positive. On the basis of the results of 16S rRNA gene sequence analyses, strain B2R-29 clearly belonged to the family , class and showed the highest similarity to EM1 (97.4 %) and to the other species of (less than 96.8 %). In the phylogenetic tree, strain B2R-29 formed a clade with EM1 and CMJ-9, indicating that is a member of the genus . Digital DNA–DNA hybridization and average nucleotide identity analyses were performed and the values between strain B2R-29 and its closely related species were less than 75.1 % and 16.9 %, respectively. The chemotaxonomic data of B2R-29 were as follows: major uniquinone, Q-8; predominant polar lipids, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol; major fatty acids, C and summed feature 3 (C 7 / C 6); predominant polyamines, putrescine, 2-hydroxyputrescine and spermidine. The DNA G+C content was 51.7 mol% from the genomic sequencing data. In accordance with the phenotypic, physiological and chemotaxonomic properties mentioned above, strain B2R-29 represents a novel species of the genus for which the name sp. nov is proposed. The type strain is B2R-29 (=CGMCC 1.13792=KCTC 72018).

Funding
This study was supported by the:
  • Science and technology program of Tibet Autonomous Region (Award XZ201901-GB-19)
    • Principle Award Recipient: DorjiPhurbu
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 31400106)
    • Principle Award Recipient: Ai-HuaLi
  • the National Natural Science Foundation of China (Award 31970004)
    • Principle Award Recipient: Ai-HuaLi
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2021-04-28
2021-05-12
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