1887

Abstract

An anaerobic, Gram-stain-positive, rod-shaped, motile and spore-forming bacterium, designated strain LBM18003, was isolated from pit clay used for making Chinese strong aroma-type liquor. Growth occurred at 20–40 °C (optimum, 30–37 °C), pH 4.5–9.5 (optimum, pH 6.5–7.0) and in the presence of 0.0–1.0 % (w/v) sodium chloride (optimum, 0 %). The predominant fatty acids were C, C, C DMA and C 3-OH, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and nine unidentified glycolipids. Phylogenetic analysis revealed that strain LBM18003 is a novel member of the family . The 16S rRNA gene sequence similarities of strain LBM18003 to its two most closely related species were less than 94.5 % for distinguishing genera, i.e. closely related to JCM 30532 (94.1 %) and DSM 107079 (93.2 %). The genome size of strain LBM18003 was 2 996 201 bp and its DNA G+C content was 48.48 mol%. Strain LBM18003 exhibited 67.8 and 68.1% pairwise-determined whole-genome average nucleotide identity values to JCM 30532 and DSM 107079, respectively; and showed 62.2 and 61.0 % the average amino acid identity values to JCM 30532 and DSM 107079, respectively; and demonstrated 46.1 and 41.5 % conserved genes to JCM 30532 and DSM 107079, respectively. The comparisons of 16S rRNA gene and genome sequences confirmed that strain LBM18003 represented a novel genus of the family . Based on morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, strain LBM18003 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is LBM18003 (=GDMCC 1.1626=JCM 33783).

Funding
This study was supported by the:
  • National First-Class Discipline Program of Light Industry Technology and Engineering (Award LITE2018-12)
    • Principle Award Recipient: WenlaiFan
  • Opening Foundation of National Engineering Research Center of Solid-state Brewing (Award 34757-2015)
    • Principle Award Recipient: YanXu
  • National Natural Science Foundation of China (NSFC) (Award 31530055)
    • Principle Award Recipient: YanXu
  • National Natural Science Foundation of China (NSFC) (Award 21706097)
    • Principle Award Recipient: CongRen
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2021-04-27
2021-05-14
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