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Volume 71, Issue 5

Description of sp. nov., a phytopathogenic actinobacterium isolated from barley seeds, causing leaf brown spot and decline Open Access

Abstract

is a Gram-stain-positive bacterium with eight subspecies, five of which have been redefined as different species on the basis of their genome sequence data. On the basis of the results of phylogenetic analysis of gene sequences, strains of members of the genus isolated from barley have been grouped in a separate clade from other species and subspecies of the genus . In this study, the biochemical, physiological, fatty acids and genetic characteristics of strains DM1 and DM3, which represented the barley isolates, were examined. On the basis of results from multi-locus sequence typing and other biochemical and physiological features, including colony colour, carbon source utilisation and enzyme activities, DM1 and DM3 are categorically differentiated from the aforementioned eight species and subspecies of the genus . Moreover, the results of genomic analysis reveal that the DNA G+C contents of DM1 and DM3 are 73.7 and 73.5 %, respectively, and the average nucleotide identity (ANI) values between DM1 and DM3 and other species and subspecies range from 90.4 to 92.0 %. The ANI value between DM1 and DM3 is 98.0 %. These results indicate that DM1 and DM3 are distinct from other known species and subspecies of the genus . Therefore, we propose a novel species, , with DM1 (=CFCC 16553 =LMG 31970) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): barley leaf brown spot and decline , barley seeds , Clavibacter and Clavibacter zhangzhiyongii sp. nov
Funding
This study was supported by the:
  • Interdepartmental funding of Genomics Research and Developmental Initiatives of Canada
    • Principle Award Recipient: Xiang(Sean) Li
  • The Basic Scientific Research Foundation of the Chinese Academy of Inspection and Quarantine (Award 2018JK004)
    • Principle Award Recipient: WenjunZhao
© 2021 Crown Copyright
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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Description of Clavibacter zhangzhiyongii sp. nov., a phytopathogenic actinobacterium isolated from barley seeds, causing leaf brown spot and decline
Int J Syst Evol Microbiol 71, 004786 (2021); https://doi.org/10.1099/ijsem.0.004786
/content/journal/ijsem/10.1099/ijsem.0.004786
/content/journal/ijsem/10.1099/ijsem.0.004786
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