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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 4

sp. nov., isolated from the intestine of the dark diving beetle , and sp. nov., isolated from the intestine of the diving beetle No Access

Abstract

A polyphasic taxonomic approach was used to characterize two novel bacterial strains, designated as HDW11 and HDW19, isolated from intestine samples of the dark diving beetle and the diving beetle , respectively. Both isolates were Gram-stain-positive, facultatively anaerobic and non-motile. Strain HDW11 grew optimally at 30 °C, pH 8 and in the presence of 1% (w/v) NaCl. Strain HDW19 grew optimally at 25 °C, pH 7 and in the presence of 0.3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences revealed that strain HDW11 is a member of the genus and is closely related to VG341 [with 97.9% 16S rRNA sequence identity and 79.1% average nucleotide identity (ANI)], and that strain HDW19 belongs to the genus and is closely related to KCTC 3621 (with 98.9% 16S rRNA sequence identity and 79.5% ANI). The major cellular fatty acids of strains HDW11 and HDW19 were C 9c and anteiso-C, respectively. The sole respiratory quinone of strain HDW11 was MK-9 (H). The major polar lipid components of strain HDW11 were diphosphatidylglycerol and phosphatidylglycerol, and the major polar lipid component of strain HDW19 was diphosphatidylglycerol. The genomic DNA G+C content of strains HDW11 and HDW19 were 72.1 and 37.2 mol%, respectively. The results of phylogenetic, phenotypic, chemotaxonomic and genotypic analyses suggest that strain HDW11 represents a novel species within the genus , and that strain HDW19 represents a novel species within the genus . We propose the name sp. nov. for strain HDW11 (=KACC 21335=KCTC 49320=JCM 33680) and the name for strain HDW19 (=KACC 21347=KCTC 43114=JCM 33684).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Brevilactibacter , Brevilactibacter coleopterorum , Cybister lewisianus , dark diving beetle , diving beetle , Hydrophilus acuminatus , Weissella and Weissella coleopterorum
Funding
This study was supported by the:
  • National Institute of Biological Resources (KR) (Award NIBR201801106)
    • Principle Award Recipient: Jin-WooBae
  • National Research Foundation (KR) (Award 2019R1A6A3A01096031)
    • Principle Award Recipient: Dong-WookHyun
  • National Research Foundation (KR) (Award NRF-2020R1A2C3012797)
    • Principle Award Recipient: Jin-WooBae
© 2021 The Authors
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2021-04-22
2024-04-20
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Brevilactibacter coleopterorum sp. nov., isolated from the intestine of the dark diving beetle Hydrophilus acuminatus, and Weissella coleopterorum sp. nov., isolated from the intestine of the diving beetle Cybister lewisianus
Int J Syst Evol Microbiol 71, 004779 (2021); https://doi.org/10.1099/ijsem.0.004779
/content/journal/ijsem/10.1099/ijsem.0.004779
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