1887

Abstract

Four unknown strains, characterized as Gram-stain-negative, strictly anaerobic, non-motile and rod-shaped, were isolated from fresh faeces of healthy humans in PR China. Pairwise sequence comparisons of the 16S rRNA genes showed that these isolates were separated into two clusters. Cluster I (strains HF-5141 and HF-106) was most closely related to XB1A (98.0–98.3 % similarity) and ATCC 8483 (97.3–97.5 %), whereas cluster II (strains HF-5287 and HF-5300) exhibited a similarity range of 96.8–97.0 % to JCM 13345, 96.7–96.9 % to MAJ27 and 96.4–96.5 % to XB1A. The DNA G+C contents of type strains HF-5141 and HF-5287 were 41.5 and 42.6 mol%, respectively. These strains had anteiso-C as the major cellular fatty acid, MK-9 and MK-11 as the predominant respiratory quinones, and phosphatidylethanolamine, aminophospholipids and phospholipids as major polar lipids, which is typical for members of the genus . However, the average nucleotide identity and digital DNA–DNA hybridization values, accompanied by different phenotypic and biochemical characteristics, distinguished them from their corresponding closest relatives as well as from other recognized members of the genus . Therefore, strains HF-5141 and HF-5287 represent two novel species in the genus , for which the names sp. nov. and sp. nov. are proposed, with HF-5141 (=CGMCC 1.16787=GDMCC 1.1591=JCM 33480) and HF-5287 (=CGMCC 1.16724=GDMCC 1.1590=JCM 33481) as type strains.

Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2019YFC1200505)
    • Principle Award Recipient: JingYang
  • National Key R&D Program of China (Award 2019YFC1200500)
    • Principle Award Recipient: JingYang
  • National Science and Technology Major Project of China (Award 2017ZX10303405-005-002)
    • Principle Award Recipient: HanZheng
  • National Science and Technology Major Project of China (Award 2017ZX10303405-002)
    • Principle Award Recipient: HanZheng
  • National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: ShanLu
  • National Science and Technology of China (Award 2017FY101202)
    • Principle Award Recipient: DongJin
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2021-05-17
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