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Volume 71, Issue 4

gen. nov., sp. nov., a novel marine bacterium isolated from a bacterial consortium that degrades polyethylene terephthalate Free

Abstract

A novel Gram-stain-negative, aerobic, gliding, rod-shaped and carotenoid-pigmented bacterium, designated A20-9, was isolated from a microbial consortium of polyethylene terephthalate enriched from a deep-sea sediment sample from the Western Pacific. Growth was observed at salinities of 1–8 %, at pH 6.5–8 and at temperatures of 10–40 °C. The results of phylogenetic analyses based on the genome indicated that A20-9 formed a monophyletic branch affiliated to the family , and the 16S rRNA gene sequences exhibited the maximum sequence similarity of 93.8 % with DSM 17368, followed by similarities of 90.4, 90.1 and 88.8 % with MCCC 1F01079, DSM 103558 and MCCC 1K02288, respectively. Its complete genome size was 4 035 598 bp, the genomic DNA G+C content was 43.2 mol%. Whole genome comparisons indicated that A20-9 and DSM 17368 shared 67.8 % average nucleotide identity, 62.7 % average amino acid identity value, 46.6% of conserved proteins and 17.8 % digital DNA–DNA hybridization identity. A20-9 contained MK-7 as the major respiratory quinone. Its major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phospatidylcholine; and the major fatty acids were iso-C (37.5 %), iso-C 3-OH (12.4 %), and summed feature 3 (Cω7 /C1ω6, 11.6 %). Combining the genotypic and phenotypic data, A20-9 could be distinguished from the members of other genera within the family and represents a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is A20-9 (=MCCC 1A17358 =KCTC 72878).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Croceimicrobium hydrocarbonivorans , deep-sea sediment and Schleiferiaceae
Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2020-9)
    • Principle Award Recipient: ZongzeShao
  • Secretaria de Desenvolvimento Econômico, Ciência, Tecnologia e Inovação (BR) (Award 16PZP001SF16)
    • Principle Award Recipient: ZongzeShao
  • COMRA program (Award No. DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
© 2021 The Authors
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2021-04-13
2024-04-23
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Croceimicrobium hydrocarbonivorans gen. nov., sp. nov., a novel marine bacterium isolated from a bacterial consortium that degrades polyethylene terephthalate
Int J Syst Evol Microbiol 71, 004770 (2021); https://doi.org/10.1099/ijsem.0.004770
/content/journal/ijsem/10.1099/ijsem.0.004770
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