1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 9

sp. nov., a novel bacterium isolated from soil under the long-term application of bifenthrin No Access

Abstract

Strain SJQ9, an aerobic bacterium isolated from a soil sample collected in Shanghai, PR China, was characterized using a polyphasic approach. It grew optimally at pH 7.0, 30–35 °C and in the presence of 1 % (w/v) NaCl. A comparative analysis of 16S rRNA gene sequences showed that strain SJQ9 fell within the genus . The closest phylogenetic relatives of strain SJQ9 were DSM 11900 (98.6 % sequence similarity) and DSM 11901 (96.4 %). Cells of the strain were Gram-stain-negative, motile, non-spore-forming, rod-shaped and positive for oxidase activity and negative for catalase. The chemotaxonomic properties of strain SJQ9 were consistent with those of the genus : the major fatty acid was summed feature 3 (C 6 and/or C 7). The isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 65.7 mol%. Strain SH9 exhibited a DNA–DNA relatedness level of 34±2 % with DSM 11900 and 28±3 % with DSM 11901. Based on the obtained data, strain SJQ9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SJQ9 (=JCM 33106=CCTCC AB 2018284).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aquabacterium soli sp. nov. , Betaproteobacteria and new taxa
Funding
This study was supported by the:
  • Shanghai Sailing Program (Award No. 17YF1416900)
    • Principle Award Recipient: LinaSun
  • Shanghai Agriculture Applied Technology Development Program, China (Award No.T20180414)
    • Principle Award Recipient: WeiguangLyu
  • SAAS Program for Excellent Research Team (Award nongkechuang 2017A-03)
    • Principle Award Recipient: WeiguangLyu
© 2021 Shanghai Academy of Agricultural Sciences, P.R.China
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004768
2021-09-28
2024-04-25
Loading full text...

Full text loading...

References

  1. Kalmbach S, Manz W, Wecke J, Szewzyk U. Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system. Int J Syst Bacteriol 1999; 49 Pt 2:769–777 [View Article] [PubMed]
     [Google Scholar]
  2. Lin M-C, Jiang S-R, Chou J-H, Arun AB, Young C-C et al. Aquabacterium fontiphilum sp. nov., isolated from spring water. Int J Syst Evol Microbiol 2009; 59:681–685 [View Article] [PubMed]
     [Google Scholar]
  3. Chen W-M, Cho N-T, Yang S-H, Arun AB, Young C-C et al. Aquabacterium limnoticum sp. nov., isolated from a freshwater spring. Int J Syst Evol Microbiol 2012; 62:698–704 [View Article] [PubMed]
     [Google Scholar]
  4. Pham VHT, Jeong S-W, Kim J. Aquabacterium olei sp. nov., an oil-degrading bacterium isolated from oil-contaminated soil. Int J Syst Evol Microbiol 2015; 65:3597–3602 [View Article] [PubMed]
     [Google Scholar]
  5. Khan IU, Habib N, Asem MD, Salam N, Xiao M et al. Aquabacterium tepidiphilum sp. nov., a moderately thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 2019; 69:337–342 [View Article] [PubMed]
     [Google Scholar]
  6. Hirose S, Tank M, Hara E, Tamaki H, Mori K et al. Aquabacterium pictum sp. nov., the first aerobic bacteriochlorophyll a-containing fresh water bacterium in the genus Aquabacterium of the class Betaproteobacteria. Int J Syst Evol Microbiol 2020; 70:596–603 [View Article] [PubMed]
     [Google Scholar]
  7. Chen W-M, Chen T-Y, Kwon S-W, Sheu S-Y. Aquabacterium lacunae sp. nov., isolated from a freshwater pond. Int J Syst Evol Microbiol 2020; 70:2888–2895 [View Article] [PubMed]
     [Google Scholar]
  8. Sun L-N, Zhang J, Gong F-F, Wang X, Hu G et al. Nocardioides soli sp. nov., a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim. Int J Syst Evol Microbiol 2014; 64:2047–2052 [View Article] [PubMed]
     [Google Scholar]
  9. Suzuki M, Nakagawa Y, Harayama S, Yamamoto S. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 2001; 51:1639–1652 [View Article] [PubMed]
     [Google Scholar]
  10. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44:992–993 [View Article] [PubMed]
     [Google Scholar]
  11. Ohta H, Hattori T. Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 1983; 49:429–446 [View Article] [PubMed]
     [Google Scholar]
  12. Sambrock J W, Russel D. Molecular Cloning: A Laboratory Manual, 3rd ed. 2001
     [Google Scholar]
  13. Lane DJ. 16S/23S rRNA Sequencing. Nucleic Acid Techniques in Bacterial Systematics 1991
     [Google Scholar]
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
     [Google Scholar]
  15. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
     [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  18. Walter MF. Toward defining the course of evolution:minimum change for a specific tree topology. Syst Zool 1971; 20:406–416
     [Google Scholar]
  19. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article] [PubMed]
     [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
     [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
     [Google Scholar]
  22. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG. Current Protocols in Molecular Biology New York: Wiley; 1995
     [Google Scholar]
  23. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
     [Google Scholar]
  24. Sasser M. MIDI technical note 101. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI, Newark 1990
     [Google Scholar]
  25. Komagata K, Susuki K. Lipid and cell-wall systematics in bacterial systematics. Methods in Microbiology 1987; 19:161–207
     [Google Scholar]
  26. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
     [Google Scholar]
  27. Wayne LG. International Committee on Systematic Bacteriology: announcement of the report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Zentralbl Bakteriol Mikrobiol Hyg A 1988; 268:433–434 [View Article] [PubMed]
     [Google Scholar]
  28. Na S-I, Kim YO, Yoon S-H, Ha S-M, Baek I et al. UBCG: up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285 [View Article] [PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004768
Loading
Aquabacterium soli sp. nov., a novel bacterium isolated from soil under the long-term application of bifenthrin
Int J Syst Evol Microbiol 71, 004768 (2021); https://doi.org/10.1099/ijsem.0.004768
/content/journal/ijsem/10.1099/ijsem.0.004768
/content/journal/ijsem/10.1099/ijsem.0.004768
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error