sp. nov., isolated from an inland brine spring in Northern England Open Access

Abstract

A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to (98.30 %), followed by (96.62 %), the two currently described species within the genus . Strain MKS20 forms white-beige-pigmented colonies and grows optimally at 28–30 °C, in 1–3 % (w/v) NaCl and at pH 7–7.5. The strain was facultatively anaerobic and showed a broader range of carbohydrate use than other species in the genus . Q-8 was the sole respiratory quinone and the major fatty acids (>10 %) were summed feature 3 (C ω6 and/or C ω7) and C. The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyglycerol and several unidentified lipids. The G+C content of the genomic DNA was 44.2 mol%. Average nucleotide identity and DNA–DNA hybridization data were consistent with assignment to a separate species. Based on the phylogenetic and genomic-based analyses, as well as physiological and biochemical characteristics, we propose that strain MKS20 (=DSM 109936, MCCC 1K04071) represents a new species of the genus , with the name sp. nov.

Funding
This study was supported by the:
  • Fundo para o Desenvolvimento das Ciências e da Tecnologia
    • Principle Award Recipient: AndréAntunes
  • Horizon 2020 Framework Programme (Award grant agreement No 654008; access to DSMZ was funded by the European Marine Biological Research Infrastructure Cluster (EMBRIC) Transnational Access Program (EMBRIC TNA Project 5667))
    • Principle Award Recipient: AndréAntunes
  • Society for Applied Microbiology (Award Society for Applied Microbiology’s Student Placement Scholarship)
    • Principle Award Recipient: MatthewKelbrick
  • Edge Hill University (Award RIF Project 1ANTUN16- EDEN)
    • Principle Award Recipient: AndréAntunes
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2021-03-19
2024-03-28
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