Full text loading...
Abstract
A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20T) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to Motilimonas eburnea (98.30 %), followed by Motilimonas pumila (96.62 %), the two currently described species within the genus Motilimonas . Strain MKS20T forms white-beige-pigmented colonies and grows optimally at 28–30 °C, in 1–3 % (w/v) NaCl and at pH 7–7.5. The strain was facultatively anaerobic and showed a broader range of carbohydrate use than other species in the genus Motilimonas . Q-8 was the sole respiratory quinone and the major fatty acids (>10 %) were summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and C16 : 0. The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyglycerol and several unidentified lipids. The G+C content of the genomic DNA was 44.2 mol%. Average nucleotide identity and DNA–DNA hybridization data were consistent with assignment to a separate species. Based on the phylogenetic and genomic-based analyses, as well as physiological and biochemical characteristics, we propose that strain MKS20T (=DSM 109936T, MCCC 1K04071T) represents a new species of the genus Motilimonas , with the name Motilimonas cestriensis sp. nov.
- Received:
- Accepted:
- Published Online:
Funding
-
Fundo para o Desenvolvimento das Ciências e da Tecnologia
- Principle Award Recipient: AndréAntunes
-
Horizon 2020 Framework Programme
(Award grant agreement No 654008; access to DSMZ was funded by the European Marine Biological Research Infrastructure Cluster (EMBRIC) Transnational Access Program (EMBRIC TNA Project 5667))
- Principle Award Recipient: AndréAntunes
-
Society for Applied Microbiology
(Award Society for Applied Microbiology’s Student Placement Scholarship)
- Principle Award Recipient: MatthewKelbrick
-
Edge Hill University
(Award RIF Project 1ANTUN16- EDEN)
- Principle Award Recipient: AndréAntunes