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Volume 71, Issue 3

sp. nov., a new β-1,3-glucan-decomposing bacterium isolated from anoxic soil subjected to biological soil disinfestation Free

Abstract

Biological soil disinfestation (BSD) or reductive soil disinfestation (RSD) is a bioremediation method used to suppress or eliminate soil-borne plant pathogens by stimulating activities of indigenous anaerobic bacteria of the soil. An anaerobic bacterial strain (TW1) was isolated from an anoxic soil sample subjected to the BSD treatment and comprehensively characterized. Cells of the strain were Gram-stain-positive, slightly curved and motile rods producing terminal spores. The strain was aerotolerant. Strain TW1 was saccharolytic and produced acetate, butyrate, H and CO as fermentation end products. Strain TW1 decomposed β-1,3-glucan (curdlan and laminarin) and degraded mycelial cells of an ascomycete plant pathogen. Major cellular fatty acids of strain TW1 were C, C dimethylacetal (DMA), C aldehyde and C DMA. Strain TW1 made a group on the phylogenetic tree constructed based on 16S rRNA gene sequences with species such as (96.3 %) and (96.0 %). Whole genome analysis of strain TW1 showed that the total length of the genome was 5.28 Mb with the DNA G+C content of 31.3 mol%. The average nucleotide identity (ANIb) between strain TW1 and was 71.2 %. Presence of the genes encoding laminarinase or GH16 β-glucosidase was confirmed from the genome analysis of strain TW1. Based on the genomic, phylogenetic and phenotypic properties obtained, we propose strain TW1 should be assigned in the genus in the family as sp. nov. The type strain TW1 (=NBRC 112097=DSM 110791).

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  • Accepted:
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Keyword(s): anti-fungal enzyme , biological or reductive soil disinfestation , Clostridia , Fusarium oxysporum , laminarinase and β-1,3-glucanase
Funding
This study was supported by the:
  • Ministry of Agriculture, Forestry and Fisheries (Award 27016C)
    • Principle Award Recipient: AtsukoUeki
© 2021 The Authors
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2021-03-18
2024-04-19
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Clostridium fungisolvens sp. nov., a new β-1,3-glucan-decomposing bacterium isolated from anoxic soil subjected to biological soil disinfestation
Int J Syst Evol Microbiol 71, 004761 (2021); https://doi.org/10.1099/ijsem.0.004761
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