1887

Abstract

A novel Gram-stain-negative, facultative aerobic and rod-shaped bacterium, designated as MKL-01 and isolated from the blood of immunocompromised patient, was genotypically and phenotypically characterized. The colonies were found to be creamy yellow and convex. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain MKL-01 was most closely related to LMG 5886, present within a large cluster in the genus . The genome sequence of strain MKL-01 showed the highest average nucleotide identity value of 92.1 % and digital DNA–DNA hybridization value of 44.8 % with the closely related species LMG 5886. The genome size of the isolate was 5 750 268 bp, with a G+C content of 67.87 mol%. The strain could grow at 10–45 °C (optimum, 37–40 °C), in the presence of 0–10 % (w/v) NaCl (optimum, 0.5%) and at pH 6.0–10.0 (optimum, pH 7.0). Strain MKL-01 was positive for catalase and negative for oxidase. The major fatty acids were C, summed feature 3 (C 7/C 6 and/or C 6/C 7) and summed feature 8 (C 7 and/or C 6). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and one unidentified polar lipid. Moreover, strain MKL-01 contained ubiquinone Q-8 as the sole respiratory quinone. Based on its molecular, phenotypic and chemotaxonomic properties, strain MKL-01 represents a novel species of the genus ; the name sp. nov. is proposed for this strain. The type strain is MKL-01.

Funding
This study was supported by the:
  • National Research Foundation of Korea (Award No.2020R1A5A1018052)
    • Principle Award Recipient: Mi-KyungLee
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2021-04-13
2021-05-17
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