1887

Abstract

Two Gram-stain-negative, aerobic, rod-shaped bacteria, polar flagellated, designated strains SM2066 and SM1966, were respectively isolated from the surfaces of and macroalgae collected off the coastal areas of Rongcheng, PR China. Strain SM2066 grew at 8–37 °C and with 0.5–7.0 % (w/v) NaCl, while strain SM1966 grew at 5–30 °C and with 0.5–8.5% (w/v) NaCl. Both of them reduced nitrate to nitrite and required Na for growth but neither of them hydrolysed starch and DNA. Phylogenetic analysis based on 16S rRNA gene and single-copy orthologous cluster sequences revealed that both strains SM2066 and SM1966 were affiliated with the genus but formed distinct phylogenetic branches from known species, respectively sharing the highest 16S rRNA gene sequence similarities with type strains of (97.9 %) and (96.7 %). The digital DNA–DNA hybridization and average nucleotide identity values between strains SM2066 and SM1966 and type strains of closely related species were all below 22.9 and 79.9 mol%, respectively. The major fatty acids of the two strains were summed feature 3 (C 6/C 7), summed feature 8 (C 7) and C, with their predominant polar lipids being phosphatidylethanolamine and phosphatidylglycerol, and their sole respiratory quinone being Q-8. The genomic DNA G+C contents of strains SM2066 and SM1966 determined from genomic sequences were 40.3 and 41.6 mol%, respectively. On the basis of the polyphasic evidence presented in this study, strains SM2066 and SM1966 are considered to represent two novel species within the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains are SM2066 (=MCCC 1K04390= KCTC 82372) and SM1966 (=MCCC 1K04387= KCTC 72848), respectively.

Funding
This study was supported by the:
  • Taishan Scholars Program of Shandong Province (Award tspd20181203)
    • Principle Award Recipient: Yu-ZhongZhang
  • National Science Foundation of China (Award 42076151)
    • Principle Award Recipient: Xi-YingZhang
  • National Science Foundation of China (Award U1706207)
    • Principle Award Recipient: Yu-ZhongZhang
  • National Science Foundation of China (Award 31870052)
    • Principle Award Recipient: Xiu-LanChen
  • Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (Award 2019JZZY010817)
    • Principle Award Recipient: Yu-ZhongZhang
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2021-03-04
2022-05-22
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