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Abstract
Two Gram-stain-positive strains, c9Ua_26_MT and c11Ua_112_MT, were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus Limosilactobacillus . Phylogenetic analysis based on pheS gene sequences and core genomes showed that each strain formed a separated branch and are closest to Limosilactobacillus vaginalis DSM 5837T. The average nucleotide identity (ANI) and Genome-to-Genome Distance Calculator (GGDC) values between c9Ua_26_MT and the closest relative DSM 5837T were 90.7 and 42.9 %, respectively. The ANI and GGDC values between c11Ua_112_MT and the closest relative DSM 5837T were 91.2 and 45.0 %, and those among the strains were 92.9% and 51,0 %, respectively. The major fatty acids were C12 : 0 (40.2 %), C16 : 0 (26.7 %) and C18 : 1 ω9c (17.7 %) for strain c9Ua_26_MT, and C18 : 1 ω9c (38.0 %), C16 : 0 (33.3 %) and C12 : 0 (17.6 %) for strain c11Ua_112_MT. The genomic DNA G+C content of strains c9Ua_26_MT and c11Ua_112_MT was 39.9 and 39.7 mol%, respectively. On the basis of the data presented here, strains c9Ua_26_MT and c11Ua_112_MT represent two novel species of the genus Limosilactobacillus , for which the names Limosilactobacillus urinaemulieris sp. nov. (c9Ua_26_MT=CECT 30144T=LMG 31899T) and Limosilactobacillus portuensis sp. nov. (c11Ua_112_MT=CECT 30145T=LMG 31898T) are proposed.
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Funding
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Comissão de Coordenação da Região Norte (PT)
(Award NORTH-01–0145-FEDER-000024)
- Principle Award Recipient: SvetlanaUgarcina Perovic
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Fundação para a Ciência e a Tecnologia
(Award DL57/2016/CP1346/CT0034)
- Principle Award Recipient: FilipaGrosso
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Fundação para a Ciência e a Tecnologia
(Award SFRH/BD/132497/2017)
- Principle Award Recipient: MagdalenaKsiezarek
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Applied Molecular Biosciences Unit
(Award UIDB/Multi/04378/2020)
- Principle Award Recipient: ApplicableNot
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Applied Molecular Biosciences Unit - UCIBIO
(Award UIDP/04378/2020)
- Principle Award Recipient: ApplicableNot