1887

Abstract

A Gram-stain-positive, facultatively anaerobic, endospore-forming, rod-shaped strain, AGMB 02131, which grew at 20–40 °C (optimum 30 °C), pH 3.0–11.0 (optimum pH 4.0) and in the presence of 0–18 % (w/v) NaCl (optimum 10 %), was isolated from a cow faecal sample and identified as a novel strain using a polyphasic taxonomic approach. The phylogenetic analysis based on 16S rRNA gene sequences along with the whole genome (92 core gene sets) revealed that AGMB 02131 formed a group within the genus , and showed the highest sequence similarity with DSM 28131 (96.9 %), following by DSM 18926 (96.6 %). The genome of AGMB 02131 comprised 70 contigs, the chromosome length was 4 038 965 bp and it had a 38.5 % DNA G+C content. Digital DNA–DNA hybridization revealed that AGMB 02131 displayed 21.4 % genomic DNA relatedness with the most closely related strain, DSM 18926. AGMB 02131 contains all of the conserved signature indels that are specific for members of the genus . The major cellular fatty acids (>10 %) of AGMB 02131 were Cω9, C and C. The major polar lipids present were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the phenotypic, phylogenetic, genomic and chemotaxonomic features, AGMB 02131 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AGMB 02131 (=KCTC 43221=CCTCC AB 2020077).

Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5282021)
    • Principle Award Recipient: JiyoungLee
  • National Research Foundation of Korea (Award NRF-2019M3A9F3065226)
    • Principle Award Recipient: JuHuck Lee
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/content/journal/ijsem/10.1099/ijsem.0.004721
2021-03-02
2022-05-27
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