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Volume 71, Issue 3

sp. nov. and sp. nov., isolated from raw milk in Germany Free

Abstract

In this study, nine Gram-negative, motile and rod-shaped bacteria were isolated during a Germany-wide investigation of raw milk microbiota. The strains could be differentiated from their closest relatives by phenotypic and chemotaxonomic characterization and average nucleotide identity (ANIb) values calculated from draft genome assemblies. Strains MBT-1, MBT-8, MBT-9, MBT-10, MBT-11 and MBT-12 were related to the subgroup. Isolates MBT-2, MBT-13 and MBT-14 were closely related to DSM 16299 with an ANIb of 88.2 % and a genome-to-genome distance result of 36.0 %. The G+C content of the DNA of strains MBT-1 and MBT-2 was 60.84 and 62.48 mol%, respectively. The major fatty acids were C ω7 (summed feature 3), C and C ω7 (summed feature 8). The strains were catalase-positive, while production of urease, β-galactosidase and indole were negative. Growth occurred at 4–30 °C and at pH values of pH 6.0–8.0. Based on these results, we conclude that the strains belong to two novel species, for which the names sp. nov. and sp. nov. are proposed. The type strains are MBT-1 (=DSM 111668= LMG 31954) and MBT-2 (=DSM 111761=LMG 31955).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): MALDI TOF MS , new taxa , Pseudomonas and whole genome sequencing
Funding
This study was supported by the:
  • Bundesministerium für Wirtschaft und Technologie (Award AiF 20027 N)
    • Principle Award Recipient: StefanieGieschler
© 2021 The Authors
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2021-02-23
2024-04-20
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Pseudomonas kielensis sp. nov. and Pseudomonas baltica sp. nov., isolated from raw milk in Germany
Int J Syst Evol Microbiol 71, 004717 (2021); https://doi.org/10.1099/ijsem.0.004717
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