Skip to content
1887

Abstract

was published in the in June 2012. was published in in 2016 and validated in November 2020. In the present study, the relationship between and was re-evaluated. In the 16S rRNA gene phylogeny, was closely related to and . The type strains of and shared 99.8 % 16S rRNA gene sequence similarity, 98.5 % sequence similarity, 99.9 % sequence similarity, 98.7 % average nucleotide identity (ANI) value and 88.9 % digital DNA–DNA hybridization (dDDH) value, indicating that they represent the same species. Meanwhile, although the type strains of and also shared 99.8 % 16S rRNA gene sequence similarity, 97.1 % sequence similarity and 99.6 % sequence similarity, 94.7 % ANI and 59.1 % dDDH values indicated that they represent two different species. On the basis of the results present here, we propose Ren . 2020 as a later heterotypic synonym of Morandi . 2012.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award no. 31471594)
    • Principle Award Recipient: ChunTao Gu
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004716
2021-02-23
2025-05-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/71/3/ijsem004716.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004716&mimeType=html&fmt=ahah

References

  1. De Vos P, Garrity G, Jones D, Krieg NR, Ludwig W. (editors) Taxonomic Outline of the Phylum Firmicutes. Bergey’s Manual of Systematic Bacteriology New York: Springer; 2009 pp 15–17
    [Google Scholar]
  2. Fortina MG, Ricci G, Mora D, Manachini PL. Molecular analysis of artisanal Italian cheeses reveals Enterococcus italicus sp. nov. Int J Syst Evol Microbiol 2004; 54:1717–1721 [View Article][PubMed]
    [Google Scholar]
  3. Vancanneyt M, Zamfir M, Devriese LA, Lefebvre K, Engelbeen K et al. Enterococcus saccharominimus sp. nov., from dairy products. Int J Syst Evol Microbiol 2004; 54:2175–2179 [View Article][PubMed]
    [Google Scholar]
  4. Morandi S, Cremonesi P, Povolo M, Brasca M. Enterococcus lactis sp. nov., from Italian raw milk cheeses. Int J Syst Evol Microbiol 2012; 62:1992–1996 [View Article][PubMed]
    [Google Scholar]
  5. Kadri Z, Spitaels F, Cnockaert M, Praet J, El Farricha O et al. Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk. Antonie van Leeuwenhoek 2015; 108:1257–1265 [View Article][PubMed]
    [Google Scholar]
  6. Ren X, Li M, Guo D. Enterococcus xinjiangensis sp. nov., isolated from yogurt of Xinjiang, China. Curr Microbiol 2016; 73:374–378 [View Article][PubMed]
    [Google Scholar]
  7. Oren A, Garrity GM. List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication. Int J Syst Evol Microbiol 2020; 70:5596–5600 [View Article][PubMed]
    [Google Scholar]
  8. An D, Cai S, Dong X. Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 2006; 56:2043–2048 [View Article][PubMed]
    [Google Scholar]
  9. Naser SM, Thompson FL, Hoste B, Gevers D, Dawyndt P et al. Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 2005; 151:2141–2150 [View Article][PubMed]
    [Google Scholar]
  10. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  11. Kishino H, Hasegawa M. Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. J Mol Evol 1989; 29:170–179 [View Article][PubMed]
    [Google Scholar]
  12. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. mega X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article][PubMed]
    [Google Scholar]
  13. Coil D, Jospin G, Darling AE. A5-miseq: an updated pipeline to assemble microbial genomes from illumina MiSeq data. Bioinformatics 2015; 31:587–589 [View Article][PubMed]
    [Google Scholar]
  14. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article][PubMed]
    [Google Scholar]
  15. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
    [Google Scholar]
  16. Davis JJ, Wattam AR, Aziz RK, Brettin T, Butler R et al. The PATRIC bioinformatics resource center: expanding data and analysis capabilities. Nucleic Acids Res 2020; 48:D606–D612 [View Article][PubMed]
    [Google Scholar]
  17. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
    [Google Scholar]
  18. Yoon SH, Ha S, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
    [Google Scholar]
  19. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article][PubMed]
    [Google Scholar]
  20. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  21. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  22. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
    [Google Scholar]
  23. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  24. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article][PubMed]
    [Google Scholar]
  25. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [View Article]
    [Google Scholar]
  26. Tak EJ, Kim HS, Lee JY, Kang W, Hyun D-W et al. Vagococcus martis sp. nov., isolated from the small intestine of a marten, Martes flavigula . Int J Syst Evol Microbiol 2017; 67:3398–3402 [View Article][PubMed]
    [Google Scholar]
  27. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE, USA: Microbial ID Inc; 1990
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.004716
Loading
/content/journal/ijsem/10.1099/ijsem.0.004716
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error