1887

Abstract

was published in the in June 2012. was published in in 2016 and validated in November 2020. In the present study, the relationship between and was re-evaluated. In the 16S rRNA gene phylogeny, was closely related to and . The type strains of and shared 99.8 % 16S rRNA gene sequence similarity, 98.5 % sequence similarity, 99.9 % sequence similarity, 98.7 % average nucleotide identity (ANI) value and 88.9 % digital DNA–DNA hybridization (dDDH) value, indicating that they represent the same species. Meanwhile, although the type strains of and also shared 99.8 % 16S rRNA gene sequence similarity, 97.1 % sequence similarity and 99.6 % sequence similarity, 94.7 % ANI and 59.1 % dDDH values indicated that they represent two different species. On the basis of the results present here, we propose Ren . 2020 as a later heterotypic synonym of Morandi . 2012.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award no. 31471594)
    • Principle Award Recipient: ChunTao Gu
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004716
2021-02-23
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/71/3/ijsem004716.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004716&mimeType=html&fmt=ahah

References

  1. De Vos P, Garrity G, Jones D, Krieg NR, Ludwig W. (editors) Taxonomic Outline of the Phylum Firmicutes. Bergey’s Manual of Systematic Bacteriology New York: Springer; 2009 pp 15–17
    [Google Scholar]
  2. Fortina MG, Ricci G, Mora D, Manachini PL. Molecular analysis of artisanal Italian cheeses reveals Enterococcus italicus sp. nov. Int J Syst Evol Microbiol 2004; 54:1717–1721 [View Article][PubMed]
    [Google Scholar]
  3. Vancanneyt M, Zamfir M, Devriese LA, Lefebvre K, Engelbeen K et al. Enterococcus saccharominimus sp. nov., from dairy products. Int J Syst Evol Microbiol 2004; 54:2175–2179 [View Article][PubMed]
    [Google Scholar]
  4. Morandi S, Cremonesi P, Povolo M, Brasca M. Enterococcus lactis sp. nov., from Italian raw milk cheeses. Int J Syst Evol Microbiol 2012; 62:1992–1996 [View Article][PubMed]
    [Google Scholar]
  5. Kadri Z, Spitaels F, Cnockaert M, Praet J, El Farricha O et al. Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk. Antonie van Leeuwenhoek 2015; 108:1257–1265 [View Article][PubMed]
    [Google Scholar]
  6. Ren X, Li M, Guo D. Enterococcus xinjiangensis sp. nov., isolated from yogurt of Xinjiang, China. Curr Microbiol 2016; 73:374–378 [View Article][PubMed]
    [Google Scholar]
  7. Oren A, Garrity GM. List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication. Int J Syst Evol Microbiol 2020; 70:5596–5600 [View Article][PubMed]
    [Google Scholar]
  8. An D, Cai S, Dong X. Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 2006; 56:2043–2048 [View Article][PubMed]
    [Google Scholar]
  9. Naser SM, Thompson FL, Hoste B, Gevers D, Dawyndt P et al. Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 2005; 151:2141–2150 [View Article][PubMed]
    [Google Scholar]
  10. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  11. Kishino H, Hasegawa M. Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. J Mol Evol 1989; 29:170–179 [View Article][PubMed]
    [Google Scholar]
  12. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. mega X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article][PubMed]
    [Google Scholar]
  13. Coil D, Jospin G, Darling AE. A5-miseq: an updated pipeline to assemble microbial genomes from illumina MiSeq data. Bioinformatics 2015; 31:587–589 [View Article][PubMed]
    [Google Scholar]
  14. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article][PubMed]
    [Google Scholar]
  15. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
    [Google Scholar]
  16. Davis JJ, Wattam AR, Aziz RK, Brettin T, Butler R et al. The PATRIC bioinformatics resource center: expanding data and analysis capabilities. Nucleic Acids Res 2020; 48:D606–D612 [View Article][PubMed]
    [Google Scholar]
  17. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
    [Google Scholar]
  18. Yoon SH, Ha S, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
    [Google Scholar]
  19. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article][PubMed]
    [Google Scholar]
  20. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  21. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  22. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
    [Google Scholar]
  23. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  24. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article][PubMed]
    [Google Scholar]
  25. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [View Article]
    [Google Scholar]
  26. Tak EJ, Kim HS, Lee JY, Kang W, Hyun D-W et al. Vagococcus martis sp. nov., isolated from the small intestine of a marten, Martes flavigula . Int J Syst Evol Microbiol 2017; 67:3398–3402 [View Article][PubMed]
    [Google Scholar]
  27. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE, USA: Microbial ID Inc; 1990
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004716
Loading
/content/journal/ijsem/10.1099/ijsem.0.004716
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error