@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004714, author = "Duman, Muhammed and Mulet, Magdalena and Altun, Soner and Saticioglu, Izzet Burcin and Gomila, Margarita and Lalucat, Jorge and Garcia-Valdes, Elena", title = "Pseudomonas piscium sp. nov., Pseudomonas pisciculturae sp. nov., Pseudomonas mucoides sp. nov. and Pseudomonas neuropathica sp. nov. isolated from rainbow trout", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2021", volume = "71", number = "3", pages = "", doi = "https://doi.org/10.1099/ijsem.0.004714", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004714", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "rainbow trout", keywords = "multi-locus sequence analysis", keywords = "aquaculture", keywords = "Pseudomonas", keywords = "genome analysis", eid = "004714", abstract = "Six Gram negative, motile bacteria were isolated from rainbow trout (Oncorhynchus mykiss). The 16S rRNA sequence similarity values grouped them in the Pseudomonas mandelii (strains P49, P50T, 154aT and P154b), Pseudomonas fluorescens (strain P115T) and Pseudomonas koreensis (strain P155T) phylogenetic subgroups in the genus Pseudomonas . The DNA G+C content ranged from 58.5 to 60 mol%. The strains were characterized phenotypically using API 20NE and Biolog GENIII tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and fatty acid contents. Multi-locus sequence analysis with four housekeeping gene sequences (rpoD, rpoB, gyrB and 16S rRNA) together with genome comparisons by average nucleotide identity and genome-to-genome distance calculations were performed. Results showed that the similarity values of these strains to known species type strains were lower than the thresholds established for species in the genus Pseudomonas . Based on these data, we concluded that strains P49, P50T, P115T, P154aT, P154b and P155T belonged to four novel species. The names proposed are: Pseudomonas piscium sp. nov. for strains P49 and P50T with P50T (=CECT 30175T=CCUG 74871T) as the type strain; Pseudomonas pisciculturae sp. nov. for strain P115T (CECT 30173T=CCUG 74873T); Pseudomonas mucoides sp. nov. for strains P154aT and P154b with P154aT (=CECT 30177T=CCUG 74874T) as the type strain; and Pseudomonas neuropathica sp. nov. for strain P155T (=CECT 30178T=CCUG 74875T).", }