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Abstract

Six Gram negative, motile bacteria were isolated from rainbow trout (). The 16S rRNA sequence similarity values grouped them in the (strains P49, P50, 154a and P154b), (strain P115) and (strain P155) phylogenetic subgroups in the genus . The DNA G+C content ranged from 58.5 to 60 mol%. The strains were characterized phenotypically using API 20NE and Biolog GENIII tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and fatty acid contents. Multi-locus sequence analysis with four housekeeping gene sequences (, , and 16S rRNA) together with genome comparisons by average nucleotide identity and genome-to-genome distance calculations were performed. Results showed that the similarity values of these strains to known species type strains were lower than the thresholds established for species in the genus . Based on these data, we concluded that strains P49, P50, P115, P154a, P154b and P155 belonged to four novel species. The names proposed are: sp. nov. for strains P49 and P50 with P50 (=CECT 30175=CCUG 74871) as the type strain; sp. nov. for strain P115 (CECT 30173=CCUG 74873) sp. nov. for strains P154a and P154b with P154a (=CECT 30177=CCUG 74874) as the type strain; and sp. nov. for strain P155 (=CECT 30178=CCUG 74875).

Funding
This study was supported by the:
  • Scientific and Technological Research Council of Turkey (Award TUBITAK No 118O420)
    • Principle Award Recipient: NotApplicable
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2021-02-25
2021-10-17
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