1887

Abstract

A novel mesophilic, aerotolerant anaerobic bacterium, designated JN-18, was isolated from the pit mud of a strong aromatic Chinese liquor. According to a 16S rRNA gene sequence analysis, it had the highest sequence similarity to DSM 103574 (95.69%). The G+C content of its genomic DNA was 43.39 mol%. The cells were Gram-stain-negative, slightly curved rods with flagella. Optimum growth was observed at 37 °C, pH 6.5 and without extra addition of NaCl. Strain JN-18utilized amino acids (-alanine, -arginine, -asparagine, -lysine, -methionine, -serine and -threonine), malate and pyruvate, and used -arginine and -lysine to produce acetate, butyrate, H, and CO. The major cellular fatty acids of strain JN-18 were C, C DMA and C cis-9 DMA. The carbohydrate composition of the cell wall predominantly included galactose, glucose and rhamnose. Based on its phylogenetic, phenotypic, physiological and biochemical characteristics, strain JN-18 was classified as a representative of a novel species within the genus , for which the name sp. nov. is proposed. The type strain is JN-18 (=CCAM 412=JCM 39126).

Funding
This study was supported by the:
  • the Sichuan Science and Technology Program (Award 19ZDYF2361)
    • Principle Award Recipient: NotApplicable
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2021-10-18
2024-12-12
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