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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., an actinobacterium isolated from sandy soil in Sanya Free

Abstract

A novel actinobacterium, designated strain NEAU-AAG5, was isolated from sandy soil collected from Niuwang island in Sanya, Hainan Province, PR China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-AAG5 belongs to the genus and shared highest sequence similarity with NBRC 14102 (98.8 %). Strain NEAU-AAG5 grows at 20–40 °C (optimum, 28 °C), pH 6–10 (optimum, pH 7) and has NaCl tolerance of 0–3 %. The menaquinones were identified as MK-9(H) (4.2 %), MK-9(H) (49.2 %) and MK-9(H) (46.5 %). The major fatty acids were C (31.4 %), 10-methyl C (21.3 %) and C 9 (15.7 %). The polar lipids were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylglycerol and phosphoglycolipid. The genomic DNA G+C content of strain NEAU-AAG5 based on whole genome sequences was 72.8 mol%. Digital DNA–DNA hybridization between strain NEAU-AAG5 and its closest phylogenetic neighbour, NBRC 14102, resulted in similarity value of 28.0 % (<70 %). Additionally, the average nucleotide identity was 84.2 % for NBRC 14102. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-AAG5 can be characterized to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-AAG5 (=JCM 33456=CCTCC AA 2019043).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , Actinomadura litoris sp. nov. , polyphasic approach and whole genome sequence
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 31772240)
    • Principle Award Recipient: XiangjingWang
© 2021 The Authors
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2021-02-22
2024-04-23
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Actinomadura litoris sp. nov., an actinobacterium isolated from sandy soil in Sanya
Int J Syst Evol Microbiol 71, 004708 (2021); https://doi.org/10.1099/ijsem.0.004708
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