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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

Description of p. nov., a new bacterium isolated from soil Free

Abstract

Two strains isolated from soil samples were designated as YH-JAE5 and YH-JAE2. The isolates were facultative anaerobic, Gram-stain-variable, motile, rod-shaped bacteria. Phylogenetic analysis indicated that the isolates belonged to the genus , but the 16S rRNA gene sequence similarities were <98 % when compared with other species within the genus. Analysis of gene revealed the isolates formed a sub-cluster with . The only menaquinone identified was MK-7. The two isolates contained -diaminopimelic acid within their cell wall peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipid, aminophospholipids, and lipids. The major fatty acids were C anteiso and C iso. The average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization values between isolate YH-JAE5 and the most closely related reference strain ( KCTC 3758) were 81.7, 84.8 and 23.4 %, respectively. The G+C content of the genomic DNA was 47.4 mol%. Thus, the polyphasic data revealed that YH-JAE2 (=KCTC 43239=JCM 34435) and YH-JAE5 (=KCTC 43059=JCM 33533) represent a new species. The name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): dokdo , genome , new species , Paenibacillus dokdonensis , phylogenetic and soil
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2018R1A2B5002239)
    • Principle Award Recipient: Joong-KiKook
  • Ministry of Science, ICT and Future Planning
    • Principle Award Recipient: YoungHyo Chang
  • National Research Foundation of Korea (Award 2013M3A9A5076601)
    • Principle Award Recipient: YoungHyo Chang
© 2021 The Authors
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2021-02-17
2024-04-19
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Description of Paenibacillus dokdonensis sp. nov., a new bacterium isolated from soil
Int J Syst Evol Microbiol 71, 004707 (2021); https://doi.org/10.1099/ijsem.0.004707
/content/journal/ijsem/10.1099/ijsem.0.004707
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