1887

Abstract

Four Gram-stain-positive, catalase-negative, non-spore-forming, rod-shaped bacterial strains (zg-325, zg329, dk561 and dk752) were isolated from the respiratory tract of marmot () and the faeces of Tibetan gazelle () from the Qinghai-Tibet Plateau of PR China. The results of 16S rRNA gene sequence-based phylogenetic analyses indicated that strains zg-325 and dk561 represent members of the genus , most similar to DSM 20671 and B71, respectively. The DNA G+C contents of strains zg-325 and dk561 were 71.6 and 69.3 mol%, respectively. The digital DNA–DNA hybridization values of strains zg-325 and dk561 with their most closely related species were below the 70 % threshold for species demarcation. The four strains grew best at 35 °C in air containing 5 % CO on brain heart infusion (BHI) agar with 5 % sheep blood. All four strains had Cω9 and C as the major cellular fatty acids. MK-8 and MK-9 were the major menaquinones in zg-325 while MK-10 was predominant in dk561. The major polar lipids included diphosphatidylglycerol and phosphatidylinositol. On the basis of several lines of evidence from phenotypic and phylogenetic analyses, zg-325 and dk561 represent novel species of the genus , for which the name sp. nov. and sp. nov. are proposed. The type strains are zg-325 (=GDMCC 1.1724=JCM 34091) and dk561 (=CGMCC 4.7566=JCM 33484). We also propose, on the basis of the phylogenetic results herein, the reclassification of and as comb. nov. and comb. nov., respectively.

Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2019YFC1200505)
    • Principle Award Recipient: JingYang
  • National Key R&D Program of China (Award 2019YFC1200500)
    • Principle Award Recipient: JingYang
  • National Science and Technology Major Project of China (Award 2017ZX10303405-005-002)
    • Principle Award Recipient: DongJin
  • National Science and Technology Major Project of China (Award 2017ZX10303405-002)
    • Principle Award Recipient: DongJin
  • National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: ShanLu
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2021-02-09
2021-10-24
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