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Abstract

A novel actinomycete, strain SMC 257, was isolated from a soil sample collected from mountain forest, Nan Province, Thailand. Strain SMC 257 formed tightly closed spiral spore chains on aerial mycelia. A polyphasic approach was used for the taxonomic study of this strain. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMC 257 belonged to the genus , and the closest phylogenetically related species were subsp. JCM 9946 (98.9 % 16S rRNA gene sequence similarity), TBRC 6557 (98.4 %), and subsp. JCM 3145 (98.3 %). Genome sequencing revealed a genome size of 9.76 Mbp and a G+C content of 72.3 mol%. The genome average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values that distinguished this novel strain from its closest related species were species boundary of 95–96 % and 70 %, respectively. The cell wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars were glucose, ribose, madurose and mannose. The major menaquinone was MK-9(H). The polar lipid profile consisted of phosphatidylethanolamine, hydroxyphosphatidylethanolamine, lysophosphatidylethanolamine, diphosphatidylglycerol, N-phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C 10-methyl and iso-C. Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, strain SMC 257 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is SMC 257 (=TBRC 13065=NBRC 114772).

Funding
This study was supported by the:
  • ManeeChanama , Mahidol University
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2021-02-17
2021-02-26
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