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Abstract

An obligately anaerobic, Gram-stain-negative, spore-forming, short rod-shaped bacterium, designated strain YH- T4B42, was isolated from the large intestine of a mini-pig. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus and is most closely related to KCTC 5424, KCTC 15329 and KCTC 1871, with 95.5, 92.4 and 83.0 % sequence similarity, respectively. The average nucleotide identity values for strain YH-T4B42 and the closest related strains were lower than 72 %. The G+C content of the isolate was 55.8 mol%. The cell-wall peptidoglycan was A1γ type and contained -diaminopimelic acid. The predominant fatty acids were C, C 9, C and C. The major end products of glucose fermentation were lactate, formate and acetate, with a minor amount of butyrate. Based on its phenotypic, phylogenetic and chemotaxonomic properties, a novel species, sp. nov., is proposed for strain YH-T4B42 (=KCTC 25105=NBRC 114767).

Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2018R1A2B5002239)
    • Principle Award Recipient: Joong-KiKook
  • Ministry of Science, ICT and Future Planning (Award KRIBB Research Initiative Program)
    • Principle Award Recipient: YoungHyo Chang
  • National Research Foundation of Korea (Award NRF-2013M3A9A5076601)
    • Principle Award Recipient: YoungHyo Chang
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2021-02-08
2021-10-25
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