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Volume 71, Issue 3

gen. nov., sp. nov., a novel anaerobic thermophilic chitinolytic bacterium from compost Open Access

Abstract

A novel Gram-negative, spore forming, obligately anaerobic, thermophilic, chitin-degrading bacterium, designated UUS1-1, was isolated from compost on Ishigaki Island, Japan by enrichment culturing using chitin powder as the carbon source. The strain has unique, long, hair-like rod morphological features and exhibits strong degradation activity toward crystalline chitin under thermophilic conditions. Growth of the novel strain was observed at 45–65 °C (optimum, 55 °C) and pH 6.5–7.5 (optimum, pH 7.0). In addition to chitin, the strain utilized several other carbon sources, including -acetylglucosamine, glucose, galactose, mannose, maltose, cellobiose, fructose and sucrose. The end products of chitin degradation were acetate, lactate, H and CO. Phylogenetic tree analysis based on 16S rRNA gene sequences revealed a clear affiliation of the proposed bacterium to the phylum ; the most closely related species were LX-B and DSM6193 with similarities of 90.4 and 87.8 %, respectively. The G+C content of the genomic DNA was 52.1 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of UUS1-1 and LX-B were 65.5 and 21.0 %, respectively. The cellular fatty acid composition of the strain was C, anteiso-C, C, C 3-OH and dimethyl acetal-C. Based on phenotypic, chemotaxonomic and genotypic analysis, strain UUS1-1 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is UUS1-1 (=JCM 33882=DSM 111537).

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Keyword(s): anaerobe , Capillibacterium , chitin degradation , Firmicutes , Hydrogenispora , OPB54 and thermophilic
Funding
This study was supported by the:
  • JPMJSA1801
    • Principle Award Recipient: AkihikoKosugi
  • JPMJER1502
    • Principle Award Recipient: AkihikoKosugi
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2021-03-16
2024-04-19
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Capillibacterium thermochitinicola gen. nov., sp. nov., a novel anaerobic thermophilic chitinolytic bacterium from compost
Int J Syst Evol Microbiol 71, 004693 (2021); https://doi.org/10.1099/ijsem.0.004693
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