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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 3

sp. nov., a mesophilic and moderately thermophilic cellulolytic and xylanolytic bacterium isolated from a lab-scale biogas fermenter fed with maize silage Free

Abstract

An anaerobic bacterial strain, designated MA18, was isolated from a laboratory-scale biogas fermenter fed with maize silage. Cells stained Gram-negative and performed Gram-negative in the KOH test. The peptidoglycan type was found to be A1y-meso-Dpm direct. The major cellular fatty acids were C iso, C iso, anteiso and iso DMA as well as a C unidentified fatty acid. Oxidase and catalase activities were absent. Cells were slightly curved rods, motile, formed spores and measured approximately 0.35 µm in diameter and 3.0–5.0 µm in length. When cultivated on GS2 agar with cellobiose, round, arched, shiny and slightly yellow-pigmented colonies were formed. The isolate was mesophilic to moderately thermophilic with a growth optimum between 40 and 48 °C. Furthermore, neutral pH values were preferred and up to 1.2 % (w/v) NaCl supplemented to the GS2 medium was tolerated. Producing mainly acetate and ethanol, MA18 fermented arabinose, cellobiose, crystalline and amorphous cellulose, ribose, and xylan. The genome of MA18 consists of 4 817 678 bp with a G+C content of 33.16 mol%. In the annotated protein sequences, cellulosomal components were detected. Phylogenetically, MA18 is most closely related to DSM 19573 (76.88 % average nucleotide identity of the whole genome sequence; 97.23 % 16S rRNA gene sequence similarity) and can be clustered into one clade with other species of the genus , family , class . Based on morphological, physiological and genetic characteristics, this strain represents a novel species in the genus . Therefore, the name sp. nov. is proposed. The type strain is MA18 (=DSM 109966=JCM 39124).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biomass hydrolysis , carbohydrate-active enzymes , cellulosome , Clostridia and renewable energy
Funding
This study was supported by the:
  • Bundesministerium für Ernährung und Landwirtschaft (Award 22021715)
    • Principle Award Recipient: WolfgangLiebl
  • Bundesministerium für Ernährung und Landwirtschaft (Award 22021715)
    • Principle Award Recipient: VladimirV. Zverlov
  • German Federal Ministry of Food and Agriculture (Award 22021715)
    • Principle Award Recipient: ReginaRettenmaier
© 2021 The Authors
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2021-02-08
2025-01-18
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Ruminiclostridium herbifermentans sp. nov., a mesophilic and moderately thermophilic cellulolytic and xylanolytic bacterium isolated from a lab-scale biogas fermenter fed with maize silage
Int J Syst Evol Microbiol 71, 004692 (2021); https://doi.org/10.1099/ijsem.0.004692
/content/journal/ijsem/10.1099/ijsem.0.004692
/content/journal/ijsem/10.1099/ijsem.0.004692
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